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epidermal growth factor receptor substrate 15 (EGFR substrate 15, protein Eps15, AF-1p protein. EPS15, AF1P)
Function:
1) cell growth regulation
2) regulation of mitogenic signals & control of cell proliferation
3) internalization of ligand-inducible tyrosine kinase receptors, including EGFR
4) binds AP2A2, STN2, EPN1
5) interacts with CRK via its SH3-binding sites
6) phosphorylation on Tyr-849
- role in the internalization of EGFR
7) forms heterotrimer with HGS, & either STAM1 or STAM2
Structure:
- contains 2 EF-hand domains
- contains 3 EH domains
- contains 2 UIM repeats (ubiquitin-interacting motif)
Compartment:
- cytoplasm, cell membrane
- recruited to the plasma membrane upon EGFR activation & localizes to coated pits
Expression: ubiquitously expressed
Pathology:
- chromosomal translocation t(1;11)(p32;q23) with MLL/HRX is found in acute leukemia
General
phosphoprotein
Properties
SIZE: MW = 99 kD
entity length = 896 aa
COMPARTMENT: cytoplasm
MOTIF: EH 1 {15-104}
EH 2 {128-216}
MOTIF: EF hand
SITE: 160-195
EF hand
SITE: 223-258
EH 3 {224-314}
Ser phosphorylation site {S323}
15 X 3 AA repeats of D-P-F {599-827}
MOTIF: consensus repeat {599-601}
consensus repeat {623-625}
consensus repeat {629-631}
consensus repeat {634-636}
consensus repeat {640-642}
consensus repeat {645-647}
consensus repeat {651-653}
consensus repeat {664-666}
consensus repeat {672-674}
consensus repeat {692-694}
consensus repeat {709-711}
consensus repeat {737-739}
SH3-binding site
NAME: SH3-binding site
SITE: 768-774
consensus repeat {798-800}
consensus repeat {804-806}
consensus repeat {825-827}
Tyr phosphorylation site {Y849}
FOR-PHOSPHORYLATION-BY: EGF receptor
UIM {851-870}
UIM {877-896}
Database Correlations
OMIM 600051
UniProt P42566
Pfam PF00036
References
- UniProt :accession P42566
- Atlas of Genetics and Cytogenetics in Oncology and Haematology
http://atlasgeneticsoncology.org/Genes/AF1pID11.html