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E1A-binding protein p400; EC=3.6.4.-; CAG repeat protein 32; domino homolog; hdomino; trinucleotide repeat-containing gene 12 protein; p400 kD SWI2/SNF2-related protein (EP400, CAGH32, KIAA1498, KIAA1818, TNRC12)
Function:
- component of the NuA4 histone acetyltransferase complex
- the NuA4 complex ATPase & helicase activities seem to be, at least in part, contributed by the association of RUVBL1 & RUVBL2 with EP400
- may regulate ZNF42 transcription activity
- may also participate in the formation of NuA4 related complexes which lack the KAT5/TIP60 catalytic subunit, but which include the SWI/SNF related protein SRCAP
- EP400 interacts with TRRAP, RUVBL1 & RUVBL2
- interacts with ZNF42
- interacts with PHF5A (putative)
Structure:
- belongs to the SNF2/RAD54 helicase family, SWR1 subfamily
- contains 1 helicase ATP-binding domain
- contains 1 helicase C-terminal domain
- contains 1 HSA domain
- contains 1 Myb-like domain
Compartment: nucleus (putative)
Alternative splicing: named isoforms=5
Expression: ubiquitously expressed
General
DEAD box ATP-dependent helicase
phosphoprotein
protein subunit
Properties
SIZE: entity length = 3159 aa
MW = 343 kD
COMPARTMENT: cell nucleus
MOTIF: glutamine-rich region {278-281}
MOTIF: glutamine residue (SEVERAL)
Thr phosphorylation site {T314}
Ser phosphorylation site {S315}
proline-rich region
SITE: 316-319
MOTIF: proline residue (SEVERAL)
Thr phosphorylation site {T320}
Ser phosphorylation site {S321}
glutamate-rich region {426-437}
MOTIF: glutamate residue (SEVERAL)
Ser phosphorylation site {S736}
Ser phosphorylation site {S755}
HSA {799-871}
Ser phosphorylation site {S941}
Ser phosphorylation site {S943}
Thr phosphorylation site {T945}
Interactions with RUVBL1 and RUVBL2 {951-1365}
MOTIF: helicase
NAME: helicase
SITE: 1103-1268
ATP-binding site
NAME: ATP-binding site
SITE: 1116-1123
DEAD/H box
NAME: DEAD/H box
SITE: 1219-1222
alanine-rich region {1517-1522}
MOTIF: alanine residue (SEVERAL)
Ser phosphorylation site {S1547}
Ser phosphorylation site {S1728}
Ser phosphorylation site {S1732}
Helicase C-terminal {1899-2056}
Myb-like {2360-2429}
ZNF42 interaction {2524-2789}
MOTIF: proline-rich region
SITE: 2543-2554
MOTIF: proline residue (SEVERAL)
proline-rich region
SITE: 2557-2564
MOTIF: proline residue (SEVERAL)
glutamine-rich region {2756-2784}
MOTIF: glutamine residue (SEVERAL)
Thr phosphorylation site {T2813}
proline-rich region
SITE: 2828-2834
MOTIF: proline residue (SEVERAL)
Database Correlations
OMIM 606265
UniProt Q96L91
PFAM correlations
Entrez Gene 57634
Kegg hsa:57634
References
- UniProt :accession Q96L91
- Atlas of Genetics & Cytogenetics in Oncology & Haematology
http://atlasgeneticsoncology.org/genes/EP400ID40457ch12q24.html
Component-of
NuA4 histone acetyltransferase (HAT) complex