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non-secretory ribonuclease; ribonuclease US; eosinophil-derived neurotoxin; Rnase UpI-2; ribonuclease 2; Rnase 2 (RNASE2, EDN, RNS2)
Function:
- non-secretory ribonuclease
- pyrimidine specific nuclease with a slight preference for U. cytotoxin & helminthotoxin
- selectively chemotactic for dendritic cells
- possesses a wide variety of biological activities
- a particular signal processing & glycosylation pattern may differentiate the UpI2 Rnase, found specifically in pregnant women urine, from other nonsecretory Rnases
- endonucleolytic cleavage to nucleoside 3'-phosphates & 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates
Structure:
- N-terminal region is necessary for mediating chemotactic activity
- belongs to the pancreatic ribonuclease family
Compartment:
- lysosome (probable)
- cytoplasmic granule
- matrix of eosinophil's large specific granule
Expression: liver, lung, spleen, leukocytes & body fluids
Pathology:
- EDN induces ataxia & paralysis, the neurotoxic effect known as the Gordon phenomenon
General
endoribonuclease
glycoprotein
Properties
SIZE: entity length = 161 aa
MW = 18 kD
COMPARTMENT: lysosome
cytoplasm
MOTIF: signal sequence {1-27}
histidine residue {H42}
N-glycosylation site {N44}
cysteine residue {C50}
MODIFICATION: cysteine residue {C110}
cysteine residue {C64}
MODIFICATION: cysteine residue {C123}
Substrate binding {65-69}
cysteine residue {C82}
MODIFICATION: cysteine residue {C138}
N-glycosylation site {N86}
cysteine residue {C89}
MODIFICATION: cysteine residue {C98}
N-glycosylation site {N92}
cysteine residue {C98}
MODIFICATION: cysteine residue {C89}
cysteine residue {C110}
MODIFICATION: cysteine residue {C50}
N-glycosylation site {N111}
N-glycosylation site {N119}
cysteine residue {C123}
MODIFICATION: cysteine residue {C64}
cysteine residue {C138}
MODIFICATION: cysteine residue {C82}
histidine residue {H156}
Database Correlations
OMIM 131410
UniProt P10153
Pfam PF00074
Entrez Gene 6036
Kegg hsa:6036
ENZYME 3.1.27.5
References
UniProt :accession P10153