Search
Crossover junction endonuclease EME1; MMS4 homolog; hMMS4 (EME1, MMS4)
Function:
1) interacts with MUS81 to form a DNA structure-specific endonuclease - substrate preference for branched DNA with 5'-end at the branch nick
2) substrates include
a) 3'-flap structures
b) replication forks
c) nicked Holliday junctions.
3) processing of stalled or collapsed replication forks
4) interacts with MUS81, ERCC4 & FANCM
Cofactor: Mg+2
Compartment:
- nucleus, nucleolus
- recruited to regions of DNA damage in S-phase cells
Alternative splicing: named isoforms=2
General
endodeoxyribonuclease
phosphoprotein
Properties
SIZE: entity length = 570 aa
MW = 63 kD
COMPARTMENT: cell nucleus
MOTIF: Ser phosphorylation site {S12}
Ser phosphorylation site {S15}
Ser phosphorylation site {S84}
Ser phosphorylation site {S85}
Ser phosphorylation site {S87}
Ser phosphorylation site {S111}
Ser phosphorylation site {S117}
Thr phosphorylation site {T150}
Database Correlations
OMIM 610885
UniProt Q96AY2
Pfam PF02732
Entrez Gene 146956
Kegg hsa:146956
References
UniProt :accession Q96AY2