ETS-related transcription factor Elf-3 (E74-like factor 3, epithelium-specific Ets transcription factor 1, ESE-1, epithelium-restricted Ets protein ESX, epithelial-restricted with serine box, ELF3, ERT, ESX, JEN)

Function: 1) transcriptional activator 2) binds & transactivates ETS sequences containing the consensus nucleotide core sequence GGA[AT] 3) synergistic with POU2F3 in transactivating SPRR2A promoter & with RUNX1 to transactivate ANGPT1 promoter 4) transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 & TGM3 promoters 5) represses KRT4 promoter activity 6) role in mediating vascular inflammation 7) role in epithelial cell differentiation & tumorigenesis 8) may be a critical downstream effector of the ERBB2 signaling pathway 9) may be associated with mammary gland development & involution 10) role in regulation of transcription with TATA-less promoters in preimplantation embryos, essential in preimplantation development 11) interacts with TBP 12) interacts with CREBBP & EP300 - these act as transcriptional coactivators of ELF3 & positively modulate its function 13) interacts with XRCC5/KU86 & XRCC6/KU70 - these inhibit the ability of ELF3 to bind DNA & negatively modulate its transcriptional activity 14) associated with CLND7 & POU2F3 Structure: - belongs to the ETS family - contains 1 ETS DNA-binding domain - contains 1 SAM domain (sterile alpha motif) Compartment: - cytoplasm, nucleus - localizes to cytoplasm where it transforms MCF-12A mammary epithelial cells via a novel cytoplasmic mechanism - transiently expressed & localized to the nucleus where it induces apoptosis in non-transformed breast epithelial cells MCF-10A & MCF-12A (transcription-dependent) Alternative splicing: named isoforms=2 Expression: - expressed exclusively in tissues containing a high content of terminally differentiated epithelial cells including mammary gland, colon, trachea, kidney, prostate, uterus, stomach & skin - transcriptionally regulated by ERBB2 receptor signaling in breast cancer epithelial cells - up-regulated by phorbol-12-myristate-13-acetate in bronchial epithelial cells - up-regualted by retinoic acid in MCF-7 mammary epithelial cells

Properties

SIZE: MW = 41 kD entity length = 371 aa COMPARTMENT: cytoplasm cell nucleus MOTIF: sterile alpha motif NAME: sterile alpha motif SITE: 67-138 ETS domain NAME: ETS domain SITE: 273-355

Database Correlations

OMIM 602191 UniProt P78545 PFAM correlations

References

UniProt :accession P78545