Contents

Search

Krueppel-like factor 1; erythroid krueppel-like transcription factor; EKLF (KLF1, EKLF)

Function: - transcription regulator of erythrocyte development - binds to the CACCC box in the beta-globin gene promoter & activates transcription - probably serves as a general switch factor for erythroid development - sumoylated; when sumoylated a) acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta b) represses megakaryocytic differentiation c) promotes interaction with the CDH4 subunit of the NuRD repression complex - sumoylation is promoted by PIAS1 - acetylated; can be acetylated on both Lys-274 & Lys-288 - acetylation on Lys-274 (by CBP) appears to be the major site affecting EKLF transactivation activity (putative) - phosphorylated primarily on Ser in the transactivation domain. - phosphorylation on Thr-23 is critical for the transactivation activity (putative) - interacts with CBP & EP300; the interactions enhance the transactivation activity - interacts with PCAF; the interaction a) does not acetylate EKLF b) inhibits its transactivation activity Structure: - belongs to the krueppel C2H2-type Zn+2-finger protein family - contains 3 C2H2-type Zn+2 fingers Compartment: - nucleus - colocalizes with SUMO1 in nuclear speckles (putative) Expression: - expression restricted to adult bone marrow & fetal liver - not expressed in myeloid nor lymphoid cell lines Pathology: - defects in KLF1 are the cause of blood group-Lutheran inhibitor

General

C2H2 type zinc finger protein Krueppel-like factor phosphoprotein

Properties

SIZE: entity length = 362 aa MW = 38 kD COMPARTMENT: cell nucleus MOTIF: proline-rich region SITE: 1-263 MOTIF: proline residue (SEVERAL) Thr phosphorylation site {T15} Thr phosphorylation site {T23} acidic region {61-76} MOTIF: acidic residue (SEVERAL) Zn finger C2H2-type SITE: 279-303 EFFECTOR-BOUND: Zn+2 Zn finger C2H2-type SITE: 309-333 EFFECTOR-BOUND: Zn+2 Zn finger C2H2-type SITE: 339-361 EFFECTOR-BOUND: Zn+2

Database Correlations

OMIM correlations UniProt Q13351 Pfam PF00096 Entrez Gene 10661 Kegg hsa:10661

References

  1. UniProt :accession Q13351
  2. OMIM :accession Q13351