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tripartite motif-containing protein 25; zinc finger protein 147; estrogen-responsive finger protein; Efp; RING finger protein 147 (TRIM25, EFP, RNF147, ZNF147)
Function:
- ubiquitin E3 ligase & ISG15 E3 ligase
- role in innate immune defense against viruses by mediating ubiquitination of DDX58
- mediates Lys-63-linked polyubiquitination of the DDX58 N-terminal CARD-like region
- this triggers cytosolic signal transduction leading to production of interferons in response to viral infection
- promotes ISGylation of 14-3-3 sigma
- mediates estrogen action in various target organs
- protein modification; protein ubiquitination
- auto-ISGylated
- interacts (via SPRY domain) with DDX58 (via CARD domain)
- interacts (via coiled coil) with influenza A virus NS1 protein
- this interaction specifically inhibits TRIM25 multimerization & RIM25-mediated DDX58 CARD ubiquitination, thus suppressing DDX58 signal transduction
Structure:
- the RING-type Zn+2 finger is important for ISG15 E3 ligase activity & auto-ISGylation
- auto-ISGylation negatively regulates ISG15 E3 ligase activity
- the C-terminal B30.2/SPRY domain interacts with the first N-terminal CARD domain of DDX58
- contains 1 B30.2/SPRY domain
- contains 1 RING-type Zn+2 finger
Compartment:
- cytoplasm
- colocalized with DDX58 at cytoplasmic perinuclear bodies
Expression:
- ubiquitous
- induced by interferons
General
human longevity protein
phosphoprotein
ring finger protein
Properties
SIZE: entity length = 630 aa
MW = 71 kD
MOTIF: RING-finger {13-54}
EFFECTOR-BOUND: Zn+2
FOR-BINDING-OF: DNA motif
Ser phosphorylation site {S100}
coiled coil {217-307}
MOTIF: Tyr phosphorylation site {Y278}
B30.2/SPRY {439-630}
Database Correlations
OMIM 600453
UniProt Q14258
Pfam PF00622
Entrez Gene 7706
Kegg hsa:7706
ENZYME correlations
References
- UniProt :accession Q14258
- Wikipedia; Note: TRIM25 entry
http://en.wikipedia.org/wiki/TRIM25