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transcription factor E2F2 (E2F-2, E2F2)
Function:
- transcription activator
- binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication
- the DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase
- E2F2 binds specifically to RB1 protein (cell-cycle dependent)
- component of the DRTF1/E2F transcription factor complex
- forms heterodimers with DP family members
- E2F2 complexes bind specifically hypophosphorylated retinoblastoma protein RB1
- during the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active
- binds EAPP
- phosphorylated by CDK2 & cyclin A-CDK2 in the S-phase
Structure:
- DNA-binding-domain in the central part of the molecule
- C-terminal Rb-binding domain
- belongs to the E2F/DP family
Compartment: nucleus
Expression:
- expressed in placenta > lung
- expressed in manyimmortalized cell lines derived from tumors
Pathology:
- viral oncoproteins, notably E1A, T-antigen & HPV E7, are capable of sequestering RB protein, thus releasing active E2F2 complexes
General
E2F
Properties
SIZE: entity length = 437 aa
MW = 48 kD
COMPARTMENT: cell nucleus
MOTIF: binding site
SITE: 65-105
FOR-BINDING-OF: CDK2/cyclin A complex
DNA-binding motif
SITE: 107-196
MOTIF: leucine zipper
SITE: 155-176
FOR-BINDING-VIA: leucine zipper
DEF box {160-196}
Dimerization {197-289}
Transactivation {359-437}
MOTIF: proline-rich region
SITE: 360-363
MOTIF: proline residue (SEVERAL)
binding site
SITE: 410-427
FOR-BINDING-OF: retinoblastoma-associated protein
Database Correlations
OMIM 600426
UniProt Q14209
Pfam PF02319
Entrez Gene 1870
Kegg hsa:1870
References
- UniProt :accession Q14209
- Entrez Gene :accession 1870