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E2F
E2F family of heterodimeric transcription factors originally identified through its role in transcriptional activation of the adenovirus E2 promoter [4]
Function:
- E2F forms a DNA-binding complex with DP-1 (DRTF1) & binds DNA through the E2 recognition site, 5'-TTTC[CG]CGC-3'
- E2F binds to Rb protein [3]
- cyclin-dependent kinase (CDK)-mediated phosphorylation of Rb dissociates the Rb-E2F complex, freeing E2F to facilitate transcription of S-phase specific genes [3]
Structure:
- E2F family of heterodimeric transcription factors E2F1, E2F2, E2F3 & presumably E2F4 all contain a DNA-binding-domain in the central part of the molecule & C-terminal Rb-binding domain
Pathology:
- viral oncoproteins, notably E1A, T-antigen & HPV E7, are capable of sequestering RB protein, thus releasing active E2F complexes
- E2F binds to & activates the adenovirus E2 promoter [4]
Interactions
molecular events
Related
DRTF1/E2F (DP1/E2F)
E2 recognition site
Specific
transcription factor E2F1; E2F-1; PBR3; PRB-binding protein E2F-1; retinoblastoma-associated protein 1; RBAP-1; retinoblastoma-binding protein 3; RBBP-3 (E2F1, RBBP3)
transcription factor E2F2 (E2F-2, E2F2)
transcription factor E2F3 (E2F-3, E2F3, KIAA0075)
transcription factor E2F4 (E2F-4, E2F4)
transcription factor E2F5 (E2F-5, E2F5)
transcription factor E2F6 (E2F-6, E2F6)
transcription factor E2F8 (E2F-8, E2F8)
General
immediate early activator
phosphoprotein
Figures/Diagrams
Figures/diagrams/slides/tables related to E2F
Properties
COMPARTMENT: cell nucleus
MOTIF: binding site
SITE: N-TERMINAL
FOR-BINDING-OF: CDK2/cyclin A complex
domain {CENTRAL}
MOTIF: DNA-binding motif
binding site
FOR-BINDING-OF: transcription factor DP-1
phosphorylation site
binding site
SITE: C-TERMINAL
FOR-BINDING-OF: retinoblastoma-associated protein
References
- Mudryj M, Hiebert SW, Nevins JR.
A role for the adenovirus inducible E2F transcription factor
in a proliferation dependent signal transduction pathway.
EMBO J. 1990 Jul;9(7):2179-84.
PMID: 2141565
- Xu M, Sheppard KA, Peng CY, Yee AS, Piwnica-Worms H.
Cyclin A/CDK2 binds directly to E2F-1 and inhibits the
DNA-binding activity of E2F-1/DP-1 by phosphorylation.
Mol Cell Biol. 1994 Dec;14(12):8420-31.
PMID: 7969176
- Enoch T, Norbury C.
Cellular responses to DNA damage: cell-cycle checkpoints,
apoptosis and the roles of p53 and ATM.
Trends Biochem Sci. 1995 Oct;20(10):426-30. Review.
PMID: 8533157
- Cordon-Cardo C.
Mutations of cell cycle regulators. Biological and clinical
implications for human neoplasia.
Am J Pathol. 1995 Sep;147(3):545-60. Review.
PMID: 7677168
Component-of
molecular complex