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dual specificity protein phosphatase 1, MAP kinase phosphatase 1 or CL100 phosphatase

Function: - dual specificity tyrosine/serine-threonine phosphatase - highly specific for MAP kinases - dephosphorylates MAP kinase ERK2 on both Thr-183 & Tyr-185 - inhibits DNA synthesis induced by constitutively active p21ras [3] protein tyrosine phosphate + H2O protein tyrosine + phosphate phosphoprotein + H2O a protein + phosphate Structure: - belongs to the protein-tyrosine phosphatase family - non-receptor class dual specificity subfamily - contains 1 rhodanese domain - contains 1 tyrosine-protein phosphatase domain Expression: - expressed at high levels in the lung, liver placenta & pancreas - moderate levels seen in the heart & skeletal muscle - lower levels found in the brain & kidney - induced by oxidative stress & heat shock - isolated from fibroblasts exposed to oxidative stress or heat shock - induced by mitogenic stimulation ?

Interactions

molecular events

Related

3CH134 or erp primary response gene

General

dual specificity phosphatase mitogen activated protein kinase phosphatase

Properties

SIZE: entity length = 367 aa MW = 39 kD STATE: active state MOTIF: Rhodanese {20-137} Tyrosine-protein phosphatase {175-367} MOTIF: cysteine residue {C258}

Database Correlations

OMIM 600714 UniProt P28562 PFAM correlations Entrez Gene 1843 KEGG correlations ENZYME correlations

References

  1. Sun H, Charles CH, Lau LF, Tonks NK. MKP-1 (3CH134), an immediate early gene product, is a dual specificity phosphatase that dephosphorylates MAP kinase in vivo. Cell. 1993 Nov 5;75(3):487-93. PMID: 8221888
  2. Nebreda AR. Inactivation of MAP kinases. Trends Biochem Sci. 1994 Jan;19(1):1-2. Review. PMID: 8140614
  3. Sun H, Tonks NK, Bar-Sagi D. Inhibition of Ras-induced DNA synthesis by expression of the phosphatase MKP-1. Science. 1994 Oct 14;266(5183):285-8. PMID: 7939666
  4. UniProt :accession P28562