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DREAM complex (LINC complex)
Function:
- complex exists in quiescent cells where it represses cell cycle-dependent genes
- it dissociates in S phase when LIN9, LIN37, LIN52 & LIN54 form a subcomplex that binds to MYBL2
- can both act as a transcription activator or repressor depending on the context
- in G0 phase, the complex binds to more than 800 promoters & is required for repression of E2F target genes
- in S phase, the complex selectively binds to the promoters of G2/M genes whose products are required for mitosis & participates in their cell cycle dependent activation
Structure:
- subunits: E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBBP4, RBL2, TFDP1 & TFDP2
General
molecular complex
Properties
SUBUNITS: transcription factor E2F4
MOTIF: proline-rich region
SITE: 9-12
MOTIF: proline residue (SEVERAL)
DNA-binding motif
SITE: 16-85
MOTIF: leucine zipper
SITE: 43-65
FOR-BINDING-VIA: leucine zipper
DEF box {48-85}
Dimerization {86-181}
serine-rich region {307-327}
MOTIF: serine residue (SEVERAL)
Transactivation {337-413}
MOTIF: binding site
SITE: 389-392
FOR-BINDING-OF: host cell factor
RBL1 & RBL2 interaction {390-407}
transcription factor E2F5
MOTIF: proline-rich region
SITE: 33-39
MOTIF: proline residue (SEVERAL)
DNA-binding motif
SITE: 47-118
MOTIF: leucine zipper
SITE: 76-98
FOR-BINDING-VIA: leucine zipper
DEF box {81-118}
Dimerization {119-215}
serine-rich region {233-236}
MOTIF: serine residue (SEVERAL)
Transactivation {287-346}
MOTIF: RBL2 association {323-340}
Lin-9 homolog
COMPARTMENT: cell nucleus
MOTIF: Sufficient for interaction with RB1 {1-296}
Ser phosphorylation site {S309}
coiled coil {354-413}
antolefinin
MOTIF: proline-rich region
SITE: 136-203
MOTIF: proline residue (SEVERAL)
Lin-52 homolog
MOTIF: Ser phosphorylation site {S28}
protein lin-54 homolog
COMPARTMENT: cell nucleus
MOTIF: Ser phosphorylation site {S310}
Ser phosphorylation site {S314}
CXC 1 {520-560}
CXC 2 {594-635}
Myb-related protein A
COMPARTMENT: cell nucleus
MOTIF: HTH myb-type 1 {30-81}
MOTIF: DNA-binding motif
SITE: 58-81
HTH myb-type 2 {82-137}
MOTIF: DNA-binding motif
SITE: 110-133
HTH myb-type 3 {138-188}
MOTIF: DNA-binding motif
SITE: 161-184
Transcriptional activation domain {230-295}
Negative regulatory domain {298-553}
Myb-related protein B
COMPARTMENT: cell nucleus
MOTIF: HTH myb-type 1 {26-77}
MOTIF: DNA-binding motif
SITE: 54-77
HTH myb-type 2 {78-133}
MOTIF: DNA-binding motif
SITE: 106-129
HTH myb-type 3 {134-184}
MOTIF: DNA-binding motif
SITE: 157-180
Thr phosphorylation site {T266}
Ser phosphorylation site {S393}
nuclear translocation signal {411-417}
Thr phosphorylation site {T440}
Thr phosphorylation site {T444}
Thr phosphorylation site {T487}
Thr phosphorylation site {T494}
Thr phosphorylation site {T520}
nuclear translocation signal {564-584}
MOTIF: Ser phosphorylation site {S577}
histone-binding protein RBBP4
COMPARTMENT: cell nucleus
MOTIF: acetylation site
SITE: N-TERMINUS
EFFECTOR-BOUND: acetyl
WD repeat {122-155}
WD repeat {175-206}
WD repeat {225-256}
WD repeat {271-302}
WD repeat {315-346}
WD repeat {372-403}
Rb-related p130
COMPARTMENT: cell nucleus
MOTIF: proline-rich region
SITE: 9-16
MOTIF: proline residue (SEVERAL)
alanine-rich region {17-20}
MOTIF: alanine residue (SEVERAL)
glutamate-rich region {23-26}
MOTIF: glutamate residue (SEVERAL)
Ser phosphorylation site {S413}
Pocket; binds E1A {417-1024}
MOTIF: Thr phosphorylation site {T417}
Ser phosphorylation site {S639}
Thr phosphorylation site {T642}
Ser phosphorylation site {S672}
Ser phosphorylation site {S948}
Ser phosphorylation site {S952}
Ser phosphorylation site {S966}
Ser phosphorylation site {S971}
Ser phosphorylation site {S972}
Ser phosphorylation site {S973}
Thr phosphorylation site {T974}
Ser phosphorylation site {S981}
Ser phosphorylation site {S982}
Thr phosphorylation site {T986}
glutamate-rich region {998-1001}
MOTIF: glutamate residue (SEVERAL)
Thr phosphorylation site {T1019}
STATE: active state
transcription factor DP-1
COMPARTMENT: cell nucleus
MOTIF: Ser phosphorylation site {S23}
DNA-binding motif
SITE: 113-195
MOTIF: DEF box {161-195}
Ser phosphorylation site {S183}
Dimerization {204-277}
binding site
SITE: 211-327
FOR-BINDING-OF: E2F
MOTIF: DCB1 {214-246}
proline-rich region
SITE: 250-253
MOTIF: proline residue (SEVERAL)
DCB2 {259-315}
acidic region {394-410}
MOTIF: acidic residue (SEVERAL)
transcription factor Dp-2
COMPARTMENT: cell nucleus
MOTIF: Ser phosphorylation site {S24}
Ser phosphorylation site {S42}
nuclear translocation signal {103-118}
DNA-binding motif
SITE: 129-210
MOTIF: DEF box {176-210}
Dimerization {219-292}
MOTIF: DCB1 {229-261}
DCB2 {274-330}
acidic region {432-446}
MOTIF: acidic residue (SEVERAL)
References
UniProt :accession P10243
Components
antolefinin (LIN37)
histone-binding protein RBBP4; retinoblastoma-binding protein 4; RBBP-4; retinoblastoma-binding protein p48; chromatin assembly factor 1 subunit C; CAF-1 subunit C; chromatin assembly factor I p48 subunit; CAF-I 48 kD subunit; CAF-I p48; nucleosome-remodeling factor subunit RBAP48 (RBBP4, RbAp48)
Lin-52 homolog (LIN52 C14orf46)
Lin-9 homolog (huLin-9, hLin-9, beta-subunit associated regulator of apoptosis, type 1 interferon receptor beta chain-associated protein, TUDOR gene similar protein, pRB-associated protein, LIN9, BARA, TGS)
Myb-related protein A (A-Myb, MYBL1, AMYB, p90A-myb)
Myb-related protein B (B-Myb, MYBL2, BMYB)
protein lin-54 homolog; CXC domain-containing protein 1 (LIN54 CXCDC1 KIAA2037)
Rb-related protein p130; retinoblastoma-like protein 2; RBR-2; PRB2; 130 kD retinoblastoma-associated protein; p130 (RBL2 RB2)
transcription factor DP-1; E2F dimerization partner 1; DRTF polypeptide-1; TFDP1 (DP1, DRTF1)
transcription factor Dp-2; E2F dimerization partner 2 (TFDP2, DP2)
transcription factor E2F4 (E2F-4, E2F4)
transcription factor E2F5 (E2F-5, E2F5)