DNA polymerase alpha

Function: - replicative DNA polymerase Structure: - DNA polymerase alpha:primase is a 4 subunit enzyme complex, which is assembled throughout the cell cycle - consists of the two DNA polymerase subunits POLA1 & POLA2, & the DNA primase large (PRIM2) & small (PRIM1) subunits

General

DNA polymerase multisubunit protein

Properties

COMPARTMENT: cell nucleus SUBUNITS: DNA polymerase alpha catalytic subunit MOTIF: peptide motif {124-125} Ser phosphorylation site {S186} DNA-binding motif SITE: 650-715 DNA-binding motif SITE: 1245-1376 MOTIF: Zn finger C4-type SITE: 1348-1374 EFFECTOR-BOUND: Zn+2 DNA polymerase alpha 70 kD subunit MOTIF: glutamate-rich region {101-107} MOTIF: glutamate residue (SEVERAL) threonine-rich region {115-157} MOTIF: threonine residue (SEVERAL) Thr phosphorylation site {T127} Thr phosphorylation site {T130} Ser phosphorylation site {S141} Ser phosphorylation site {S152} serine-rich region {486-489} MOTIF: serine residue (SEVERAL) primase MOTIF: active site

References

Linn S How many pols does it take to replicate nuclear DNA? Cell 66:185 1991 PMID: 1855251

Components

DNA polymerase alpha 70 kD subunit (POLA2, DNA polymerase subunit alpha B) DNA polymerase alpha catalytic subunit (POLA1, POLA, DNA polymerase subunit A) primase