DEAH box protein 9; ATP-dependent RNA helicase A; nuclear DNA helicase II; NDH II (DHX9, DDX9, LKP, NDH2)

Function: - unwinds double-stranded DNA & RNA in a 3' to 5' direction - alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids - transcriptional activator - binds MBD2, HRMT1L2/PRMT1, & RELA - may act to directly link BRCA1, CREBBP or SMN1 & the RNA polymerase II complex - can also interact with XRCC5 & with TOP2A (RNA dependent) - interaction with TOP2A is promoted by UBC9 - interaction with histone H2AFX requires phosphorylation of H2AFX on Ser-139 - may be phosphorylated by PRKDC/XRCC7 - phosphorylated upon DNA damage, probably by ATM or ATR Structure: - the MTAD domain mediates interaction with the RNA polymerase II holoenzyme - the NTD domain is necessary & sufficient for nuclear- cytoplasmic shuttling & interaction with HRMT1L2 & SMN1 - methylated: HRMT1L2 mediated methylation of undefined Arg in the NTD is required for nuclear localization - belongs to the DEAD box helicase family, DEAH subfamily - contains 2 DRBM (double-stranded RNA-binding) domains - contains 1 helicase ATP-binding domain - contains 1 helicase C-terminal domain Compartment: - cytoplasm - nucleus, nucleolus - can shuttle between nucleus & cytoplasm

Properties

SIZE: entity length = 1270 aa MW = 141 kD COMPARTMENT: cytoplasm cell nucleus MOTIF: CREBBP interaction {1-250} MOTIF: DRBM 1 {3-71} Ser phosphorylation site {S87} DRBM 2 {180-252} BRCA1 interaction {230-325} MOTIF: Ser phosphorylation site {S321} MTAD {331-380} helicase NAME: helicase SITE: 398-564 MOTIF: ATP-binding site NAME: ATP-binding site SITE: 411-418 DEAD/H box NAME: DEAD/H box SITE: 511-514 nuclear translocation signal {586-595} Helicase C-terminal {636-809} NTD {1151-1260} MOTIF: Tyr phosphorylation site {Y1234} Tyr phosphorylation site {Y1241}

Database Correlations

OMIM 603115 UniProt Q08211 PFAM correlations Entrez Gene 1660 KEGG correlations

References

UniProt :accession Q08211