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CDK2/cyclin E complex

Function: - the cyclin E/CDK2 complex controls entry into S phase - CDK2 is expressed constitutively throughout the cell cycle - association of CDK2 with cyclin A or cyclin E with subsequent dephosphorylation of Tyr15 & Thr14 on CDK2 by cdc25 phosphatase & an independently regulated phosphorylation of Thr160 activates CDK2 - CDK2/cyclin-E is maximally activated a few hours before onset of DNA synthesis - CDK2 activity is required for DNA synthesis & is responsible for phosphorylation of Rb protein & modulation of DNA-bound replication protein A

Interactions

molecular events

General

CDK/cyclin complex serine/threonine kinase

Figures/Diagrams

Figures/diagrams/slides/tables related to CDK2/cyclin E complex

Properties

STATE: active state MOTIF: S/T phosphorylation site kinase domain MOTIF: ATP-binding site NAME: ATP-binding site SUBUNITS: cyclin-dependent kinase-2 STATE: active state MOTIF: kinase domain SITE: 4-286 MOTIF: ATP-binding site NAME: ATP-binding site SITE: 10-18 Thr phosphorylation site {T14} Tyr phosphorylation site {Y15} Tyr phosphorylation site {Y19} ATP-binding site NAME: ATP-binding site SITE: 33-33 ATP-binding site NAME: ATP-binding site SITE: 81-83 ATP-binding site NAME: ATP-binding site SITE: 86-86 aspartate residue {D127} ATP-binding site NAME: ATP-binding site SITE: 129-132 ATP-binding site NAME: ATP-binding site SITE: 145-145 Thr-phosphate {T160} binding site EFFECTOR-BOUND: cyclin E BOUND-VIA: cyclin box cyclin E MOTIF: ubiquitination site (destruction box, D-box) SITE: N-TERMINAL FOR-BINDING-OF: ubiquitin cyclin box EFFECTOR-BOUND: cyclin-dependent kinase-2 BOUND-VIA: binding site Tyr-phosphate

References

  1. Dulie V, Lees E, Reed SI. Association of human cyclin E with a periodic G1-S phase protein kinase. Science. 1992 Sep 25;257(5078):1958-61. PMID: 1329201
  2. Calbiochem Apoptosis catalog

Components

cyclin E cyclin-dependent kinase-2 (CDK2)