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CDK2/cyclin A complex

Function: - the CDK2/cyclin A complex affects regulation through S-phase - CDK2 is expressed constitutively throughout the cell cycle - association of CDK2 with cyclin A or cyclin E with subsequent dephosphorylation of Tyr15 & Thr14 on CDK2 by cdc25 phosphatase & an independently regulated phosphorylation of Thr160 activates CDK2

General

CDK/cyclin complex serine/threonine kinase

Figures/Diagrams

Cyclins, CDK's & the Cell Cycle

Properties

STATE: active state MOTIF: S/T phosphorylation site kinase domain MOTIF: ATP-binding site NAME: ATP-binding site SUBUNITS: cyclin-dependent kinase-2 STATE: active state MOTIF: kinase domain SITE: 4-286 MOTIF: ATP-binding site NAME: ATP-binding site SITE: 10-18 Thr phosphorylation site {T14} Tyr phosphorylation site {Y15} Tyr phosphorylation site {Y19} ATP-binding site NAME: ATP-binding site SITE: 33-33 ATP-binding site NAME: ATP-binding site SITE: 81-83 ATP-binding site NAME: ATP-binding site SITE: 86-86 aspartate residue {D127} ATP-binding site NAME: ATP-binding site SITE: 129-132 ATP-binding site NAME: ATP-binding site SITE: 145-145 Thr-phosphate {T160} binding site EFFECTOR-BOUND: cyclin A BOUND-VIA: cyclin box cyclin A MOTIF: ubiquitination site (destruction box, D-box) SITE: N-TERMINAL FOR-BINDING-OF: ubiquitin PEST region cyclin box EFFECTOR-BOUND: cyclin-dependent kinase-2 BOUND-VIA: binding site Tyr-phosphate

References

  1. Xu et al Mol Cell Biol 14:8420 1004
  2. Cordon-Cardo C. Mutations of cell cycle regulators. Biological and clinical implications for human neoplasia. Am J Pathol. 1995 Sep;147(3):545-60. Review. PMID: 7677168
  3. Calbiochem Apoptosis catalog

Components

cyclin A cyclin-dependent kinase-2 (CDK2)