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Cullin-3; CUL-3 (CUL3 KIAA0617)

Function: - core component of multiple cullin-RING-based BCR complex(es) (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination & subsequent proteasomal degradation of target proteins - scaffold protein that may contribute to catalysis through positioning of substrate & ubiquitin-conjugating enzyme - E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit & is inhibited by the association of the deneddylated cullin with TIP120A/CAND1 (putative) - functional specificity of the BCR complex depends on the BTB domain-containing protein as the susbstrate recognition component - BCR(SPOP) is involved in ubiquitination of BMI1/PCGF4, H2AFY & DAXX, & probably GLI2 or GLI3. - BCR(KLHL9-KLHL13) controls the dynamic behavior of AURKB on mitotic chromosomes & thereby coordinates faithful mitotic progression & completion of cytokinesis - involved in ubiquitination of cyclin E & of cyclin D1 (in vitro) thus involved in regulation of G1/S transition - protein modification; protein ubiquitination - neddylated - NEDD8 is required for E3 ubiquitin-protein ligase activity of the BCR complex - deneddylated via its interaction with the COP9 signalosome (CSN complex) - forms neddylation-dependent homodimers - interacts with RBX1, RNF7, CYCE & TIP120A/CAND1 - part of the BCR(SPOP) containing SPOP - part of the probable BCR(KLHL9-KLHL13) complex with BTB domain proteins KLHL9 & KLHL13 - part of the BCR(KBTBD10) complex containing KBTBD10. - part of the BCR(ENC1) complex containing ENC1 - part of a complex consisting of BMI1/PCGF4, CUL3 & SPOP - part of a complex consisting of H2AFY, CUL3 & SPOP - interacts with KCTD5, KLHL9, KLHL13, GAN, ZBTB16, KLHL21, KLHL3, KLHL15, KLHL20, C16orf44, GMCL1L, BTBD1 - part of a complex that contains CUL3, RBX1 & GAN Structure: belongs to the cullin family Compartment: nucleus, Golgi Alternative splicing: named isoforms=3 Expression: widely expressed

General

cullin phosphoprotein

Properties

SIZE: entity length = 768 aa MW = 89 kD COMPARTMENT: cell nucleus golgi MOTIF: Tyr phosphorylation site {Y58} Ser phosphorylation site {S450} Ser phosphorylation site {S737}

Database Correlations

OMIM 603136 UniProt Q13618 PFAM correlations Entrez Gene 8452 Kegg hsa:8452

References

  1. UniProt :accession Q13618
  2. Entrez Gene :accession 8452

Component-of

BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex (KLHL20)