CUG-BP- & ETR-3-like factor 2 (CELF-2, Bruno-like protein 3, RNA-binding protein BRUNOL-3, CUG triplet repeat RNA-binding protein 2, CUG-BP2, ELAV-type RNA-binding protein 3, ETR-3, neuroblastoma apoptosis-related RNA-binding protein, hNAPOR, CUGBP2, BRUNOL3, CELF2, ETR3, NAPOR)

Function: 1) RNA-binding protein 2) role in regulation of several post-transcriptional events 3) role in pre-mRNA alternative splicing, mRNA translation & stability 4) mediates exon inclusion &/or exclusion in pre-mRNA subject to tissue-specific & developmentally regulated alternative splicing 5) specifically activates exon 5 inclusion of TNNT2 in embryonic, but not adult, skeletal muscle 6) activates TNNT2 exon 5 inclusion by antagonizing repressive effect of PTB 7) role as both an activator & repressor of a pair of coregulated exons: - promotes inclusion of the smooth muscle (SM) exon but exclusion of non-muscle exon in actinin pre-mRNAs 8) promotes inclusion of exon 21 & exclusion of exon 5 of NMDA receptor R1 pre-mRNA 9) role in apoB RNA editing activity 10) increases COX2 mRNA stability & inhibits COX2 mRNA translation in epithelial cells after radiation injury 11) modulates cellular apoptosis by regulating COX2-mediated prostaglandin E2 (PGE2) expression 12) binds to (CUG)n triplet repeats in 3'-UTR of transcripts such as DMPK 13) binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the TNNT2 alternative exon 5 14) binds preferentially to UG-rich sequences, in particular UG repeat & UGUU motifs 15) binds to apoB mRNA, specifically to AU-rich sequences located immediatly upstream of the edited cytidine 16) binds AU-rich sequences in the 3'-UTR of COX2 mRNA 17) binds to intronic RNA element responsible for the silencing of exon splicing 18) found in a complex with APOBEC1 & ACF Structure: - belongs to the CELF/BRUNOL family - contains 3 RRM domains (RNA recognition motif) Compartment: - nucleus, cytoplasm - accumulates in cytoplasm after ionizing radiation - colocalizes with APOBEC1 & ACF - RNA-binding activity is detected in both nuclear & cytoplasmic compartments Alternative splicing: named isoforms=5 Expression: - expressed in frontal cortex - isoform 1 isexpressed in brain & lung - isoform 2 is expressed in heart, brain, placenta, lung, liver, kidney, skeletal muscle & pancreas - isoform 4 is expressed in heart, lung, skeletal muscle, kidney & pancreas - isoform 1 is expressed in fetal brain - isoform 2 is expressed in fetal heart, brain, thymus, lung, liver, skeletal muscle, kidney & spleen - isoform 4 is expressed in fetal heart, brain, thymus, lung & skeletal muscle

Properties

SIZE: MW = 54 kD entity length = 508 aa COMPARTMENT: cytoplasm cell nucleus MOTIF: RNA binding, exon inclusion NAME: RNA binding, exon inclusion SITE: 1-283 MOTIF: RNP motif NAME: RNP motif SITE: 40-123 FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif NAME: ribonucleoprotein-1 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif ribonucleoprotein-2 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif RNP motif NAME: RNP motif SITE: 132-212 FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif NAME: ribonucleoprotein-1 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif ribonucleoprotein-2 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif alanine-rich region {284-397} MOTIF: alanine residue (SEVERAL) binding site SITE: 357-508 FOR-BINDING-OF: NECESSARY FOR RNA MOTIF: RNP motif NAME: RNP motif SITE: 423-501 FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif NAME: ribonucleoprotein-1 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif ribonucleoprotein-2 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif

Database Correlations

OMIM 602538 UniProt O95319 Pfam PF00076

References

UniProt :accession O95319