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cleavage stimulation factor
Function:
- one of multiple factors required for polyadenylation & 3'-end cleavage of mammalian pre-mRNAs
Structure:
- subunits:
a) CSTF1 (50 kD subunit),
b) CSTF2 (64 kD subunit)
c) CSTF3 (77 kD subunit)
General
multisubunit protein
Properties
SUBUNITS: cleavage stimulation factor 50 kD subunit
MOTIF: Hydrophobic {14-35}
WD repeat {106-145}
WD repeat {171-210}
WD repeat {215-254}
WD repeat {260-301}
WD repeat {303-343}
Tyr phosphorylation site {Y367}
WD repeat {395-430}
cleavage stimulation factor 64 kD subunit
MOTIF: RNP motif
NAME: RNP motif
SITE: 16-94
FOR-BINDING-OF: ribonucleic acid
MOTIF: ribonucleoprotein-1 motif
NAME: ribonucleoprotein-1 motif
FOR-BINDING-OF: ribonucleic acid
MOTIF: ribonucleoprotein-1 motif
ribonucleoprotein-2 motif
ribonucleoprotein-2 motif
FOR-BINDING-OF: ribonucleic acid
MOTIF: ribonucleoprotein-1 motif
ribonucleoprotein-2 motif
CSTF3/SYMPK interaction {108-248}
MOTIF: Ser phosphorylation site {S154}
proline-rich region
SITE: 198-409
MOTIF: proline residue (SEVERAL)
M-E-A-R-[AG] repeats {410-469}
MOTIF: consensus repeat {410-414}
consensus repeat {415-419}
consensus repeat {420-424}
consensus repeat {425-429}
consensus repeat {430-434}
consensus repeat {435-439}
consensus repeat {440-444}
consensus repeat {445-449}
consensus repeat {450-454}
consensus repeat {455-459}
consensus repeat {460-464}
consensus repeat {465-469}
proline-rich region
SITE: 470-526
MOTIF: proline residue (SEVERAL)
RPO2TC1 interaction {514-577}
MOTIF: Ser phosphorylation site {S518}
Ser phosphorylation site {S524}
cleavage stimulation factor 77 kD subunit
MOTIF: HAT {45-77}
HAT {79-110}
HAT {117-152}
HAT {163-196}
HAT {221-261}
HAT {271-303}
HAT {319-352}
HAT {354-387}
HAT {458-494}
proline-rich region
SITE: 559-626
MOTIF: proline residue (SEVERAL)
Ser phosphorylation site {S691}
References
- Kleiman FE & Manley JL
Functional interaction of BRCA1-associated BARD1 with
polyadenylation factor CstF-50
Science 285:1576-9 1999
PMID: 10477523
Components
cleavage stimulation factor 50 kD subunit; CSTF 50 kD subunit; CF-1 50 kD subunit; CstF-50 (CSTF1)
cleavage stimulation factor 64 kD subunit; CSTF 64 kD subunit; CstF-64; CF-1 64 kD subunit (CSTF2)
cleavage stimulation factor 77 kD subunit; CSTF 77 kD subunit; CstF-77; CF-1 77 kD subunit (CSTF3)