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Comet assay (comet tail moment)
A measure of DNA damage.
Assay detects alkali-labile lesions in cells sustaining DNA damage, most likely:
1) single-strand breaks
2) double-strand breaks
3) apurinic/apyrimidinic sites
Electrophoresis performed on single cells.
Calculations based on density of comet tail & distance from center of nucleus*
* integrated density in comet tail multiplied by distance from center of nucleus to center of mass of tail software free of charge [2]
General
DNA damage
References
- Deutsch WA, Kukreja A, Shane B, Hegde V.
Phenobarbital, oxazepam and Wyeth 14,643 cause DNA damage as
measured by the Comet assay.
Mutagenesis. 2001 Sep;16(5):439-42.
PMID: 11507244
- Herbert M Geller
http://www2.umdnj.edu/geller/lab/comet.htm
- Singh NP, McCoy MT, Tice RR, Schneider EL.
A simple technique for quantitation of low levels of
DNA damage in individual cells.
Exp Cell Res. 1988 Mar;175(1):184-91.
PMID: 3345800