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dual specificity protein kinase CLK1; CDC-like kinase 1 (CLK1, CLK)
Function:
- dual specificity protein kinase
- phosphorylates both Ser/Thr & Tyr-containing substrates
- phosphorylates Ser- & Arg-rich (SR) proteins of the spliceosomal complex & may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing
- phosphorylates SRSF1, SRSF3 & PTPN1
- regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells & adenovirus E1A pre-mRNA
- regulates splicing of its own pre-mRNA according to its kinase activity
- increased expression of the catalytically active form influences splicing to generate the catalytically inactive splicing variant lacking the kinase domain
- autophosphorylates on Ser, Thr & Tyr
- interacts with PPIG & UBL5
Inhibition:
- leucettine L41 inhibits its kinase activity & affects the regulation of alternative splicing mediated by phosphorylation of SR proteins (putative)
Structure:
- belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family, lammer subfamily
- contains 1 protein kinase domain
Compartment: nucleus
Alternative splicing:
- named isoforms=2
- one isoform lacks the kinase domain
- at least one isoform may be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay
Expression: endothelial cells
General
protein kinase CLK (cdc-like kinase)
Properties
SIZE: entity length = 484 aa
MW = 57 kD
COMPARTMENT: cell nucleus
STATE: active state
MOTIF: Ser phosphorylation site {S37}
Ser phosphorylation site {S61}
Thr phosphorylation site {T138}
Ser phosphorylation site {S140}
kinase domain
SITE: 161-477
MOTIF: ATP-binding site
NAME: ATP-binding site
SITE: 167-175
ATP-binding site
NAME: ATP-binding site
SITE: 191-191
aspartate residue {D288}
Thr phosphorylation site {T330}
Thr phosphorylation site {T338}
Ser phosphorylation site {S341}
Thr phosphorylation site {T342}
Database Correlations
OMIM 601951
UniProt P49759
Pfam PF00069
Entrez Gene 1195
Kegg hsa:1195
ENZYME 2.7.12.1
References
UniProt :accession P49759