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keratin, type II cytoskeletal 8; cytokeratin-8; CK-8; keratin-8; K8 (KRT8 CYK8)
Function:
- Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle
- phosphorylation on Ser is enhanced during EGF stimulation & mitosis
- Ser-74 phosphorylation plays a role in keratin filament reorganization
- keratin-8 associates with keratin-18
- associates with KRT20
- when associated with KRT19, interacts with DMD
- interacts with TCHP
- interacts with HCV core protein & PNN
Structure:
- O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues
- heterotetramer of 2 type 1 & 2 type 2 keratins
- belongs to the intermediate filament family
Compartment: cytoplasm
Expression:
- observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin & spectrin
- expressed in gingival mucosa & hard palate of the oral cavity
Pathology:
- defects in KRT8 are a cause of cryptogenic cirrhosis
- diagnostic pathology: recognized by CAM 5.2 antibody
Related
p63+smooth muscle myosin heavy chain+cytokeratin 8 Ag in tissue
General
cytokeratin, epithelial keratins or soft alpha-keratins
phosphoprotein
Properties
CONFIGURATION: oligomer
SIZE: entity length = 483 aa
MW = 54 kD
COMPARTMENT: cytoplasm
MOTIF: Head {2-90}
MOTIF: serine-rich region {9-49}
MOTIF: serine residue (SEVERAL)
Ser phosphorylation site {S9}
Ser phosphorylation site {S13}
Ser phosphorylation site {S21}
Ser phosphorylation site {S22}
Ser phosphorylation site {S24}
Thr phosphorylation site {T26}
Ser phosphorylation site {S27}
Ser phosphorylation site {S34}
Ser phosphorylation site {S36}
Ser phosphorylation site {S37}
Ser phosphorylation site {S43}
Ser phosphorylation site {S44}
Ser phosphorylation site {S74}
Rod {91-398}
MOTIF: Coil 1A {91-126}
Linker 1 {127-143}
Coil 1B {144-235}
Tyr phosphorylation site {Y204}
Linker 12 {236-259}
Ser phosphorylation site {S251}
Ser phosphorylation site {S253}
Ser phosphorylation site {S258}
Coil 2 {260-398}
Necessary for interaction with PNN {261-382}
Tyr phosphorylation site {Y267}
Ser phosphorylation site {S274}
Ser phosphorylation site {S330}
glycine residue {342}
Tail {399-483}
MOTIF: Ser phosphorylation site {S400}
Ser phosphorylation site {S410}
Ser phosphorylation site {S417}
Ser phosphorylation site {S424}
Ser phosphorylation site {S432}
Ser phosphorylation site {S451}
Ser phosphorylation site {S475}
Ser phosphorylation site {S478}
Database Correlations
OMIM correlations
MORBIDMAP 148060
UniProt P05787
Pfam PF00038
Entrez Gene 3856
Kegg hsa:3856
References
- UniProt :accession P05787
- Human intermediate Filament mutation Database
http://www.interfil.org
- Entrez Gene :accession 3856