carbohydrate sulfotransferase 8; galNAc-4-O-sulfotransferase 1; galNAc-4-ST1; galNAc4ST-1; N-acetylgalactosamine-4-O-sulfotransferase 1 (CHST8)

Function: - catalyzes transfer of sulfate to position 4 of non-reducing N-acetylgalactosamine (galNAc) residues in both N-glycans & O-glycans - required for biosynthesis of glycoprotein hormones lutropin & thyrotropin, by mediating sulfation of their carbohydrate structures - only active against terminal galNAcbeta1,galNAcbeta - not active toward chondroitin Kinetic parameters: - KM=10 uM for carbonic anhydrase VI - pH dependence: optimum pH is 7.2 Structure: belongs to the sulfotransferase 2 family Compartment: - Golgi membrane - single-pass type 2 membrane protein (putative) Expression: - predominantly expressed in pituitary gland - in brain, expressed in pituitary gland, cerebellum, medulla oblongata, pons, thalamus & spinal cord - expressed at lower level in lung, spleen, adrenal gland, placenta, prostate, testis, mammary gland & trachea Pathology: - down-regulation of CHST8 (17-fold) in prion-infected cells

Properties

SIZE: entity length = 424 aa MW = 49 kD COMPARTMENT: golgi MOTIF: transmembrane domain {11-31} N-glycosylation site {N128} N-glycosylation site {N294} N-glycosylation site {N367} N-glycosylation site {N415}

Database Correlations

OMIM 610190 UniProt Q9H2A9 Pfam PF03567 Entrez Gene 64377 Kegg hsa:64377

References

UniProt :accession Q9H2A9