chromodomain-helicase-DNA-binding protein 4; CHD-4; ATP-dependent helicase CHD4; Mi-2 autoantigen 218 kD protein; Mi2-beta (CHD4)

Function: - component of the histone deacetylase NuRD complex - interacts directly with IKFZ1 in the NuRD complex - interacts with TRIM27 - interacts with KLF1 - interaction depends on sumoylation of KLF1, & leads to its transcriptional repression (putative) - interacts with ZGPAT - interacts with PCNT - part of a complex containing ATR & HDAC2 - phosphorylated upon DNA damage, probably by ATM or ATR ATP + H₂O = ADP + phosphate Structure: - belongs to the SNF2/RAD54 helicase family - contains 2 chromo domains - contains 1 helicase ATP-binding domain - contains 1 helicase C-terminal domain - contains 2 PHD-type Zn⁺² fingers Compartment: - nucleus, cytoplasm, cytoskeleton, centrosome - associates with centrosomes in interphase Alternative splicing: named isoforms=2 Pathology: - one of the main antigens reacting with anti-MI-2 positive sera of dermatomyositis

General

chromodomain-helicase-DNA-binding protein phosphoprotein zinc finger protein

Properties

SIZE: entity length = 1912 aa MW = 218 kD COMPARTMENT: cytoplasm cell nucleus MOTIF: lysine-rich region {50-59} MOTIF: lysine residue (SEVERAL) glutamate-rich region {94-98} MOTIF: glutamate residue (SEVERAL) Ser phosphorylation site {S103} lysine-rich region {114-119} MOTIF: lysine residue (SEVERAL) glutamate-rich region {134-138} MOTIF: glutamate residue (SEVERAL) alanine-rich region {227-235} MOTIF: alanine residue (SEVERAL) proline-rich region SITE: 248-252 MOTIF: proline residue (SEVERAL) lysine-rich region {350-354} MOTIF: lysine residue (SEVERAL) Zn finger PHD-type NAME: Zn finger PHD-type SITE: 370-417 EFFECTOR-BOUND: Zn+2 Ser phosphorylation site {S428} Zn finger PHD-type NAME: Zn finger PHD-type SITE: 449-496 EFFECTOR-BOUND: Zn+2 chromodomain NAME: chromodomain SITE: 494-594 FOR-BINDING-OF: heterochromatin MOTIF: Ser phosphorylation site {S515} Thr phosphorylation site {T517} Thr phosphorylation site {T529} Ser phosphorylation site {S531} chromodomain NAME: chromodomain SITE: 622-697 FOR-BINDING-OF: heterochromatin helicase NAME: helicase SITE: 738-922 MOTIF: ATP-binding site NAME: ATP-binding site SITE: 751-758 DEAD/H box NAME: DEAD/H box SITE: 873-876 Helicase C-terminal {1054-1203} glutamate-rich region {1294-1301} MOTIF: glutamate residue (SEVERAL) Ser phosphorylation site {S1308} Ser phosphorylation site {S1349} Ser phosphorylation site {S1370} Ser phosphorylation site {S1531} Ser phosphorylation site {S1535} Ser phosphorylation site {S1537} Thr phosphorylation site {T1540} Thr phosphorylation site {T1542} Thr phosphorylation site {T1545} Thr phosphorylation site {T1549} Thr phosphorylation site {T1553} Ser phosphorylation site {S1570} Ser phosphorylation site {S1576} PCNT interaction {1577-1912} MOTIF: Ser phosphorylation site {S1602} glutamate-rich region {1665-1668} MOTIF: glutamate residue (SEVERAL)

Database Correlations

OMIM 603277 UniProt Q14839 PFAM correlations Entrez Gene 1108 Kegg hsa:1108 ENZYME 3.6.4.12

References

UniProt :accession Q14839