chromodomain-helicase-DNA-binding protein 3; CHD-3; ATP-dependent helicase CHD3; Mi-2 autoantigen 240 kD protein; Mi2-alpha; zinc finger helicase; hZFH (CHD3)

Function: - component of the histone deacetylase NuRD complex - interacts with TRIM28 & SERBP1 - interacts (via its C-terminal) with HABP4 - interacts with PCNT; - interaction regulates centrosome integrity ATP + H₂O = ADP + phosphate Structure: - belongs to the SNF2/RAD54 helicase family - contains 2 chromo domains - contains 1 helicase ATP-binding domain - contains 1 helicase C-terminal domain - contains 2 PHD-type Zn⁺² fingers Compartment: - nucleus, cytoplasm, cytoskeleton - associates with centrosomes in interphase & mitosis Alternative splicing: named isoforms=2 Expression: widely expressed Pathology: - one of the main antigens reacting with anti-MI-2 positive sera of dermatomyositis Laboratory: -

General

chromodomain-helicase-DNA-binding protein phosphoprotein zinc finger protein

Properties

SIZE: entity length = 2000 aa MW = 227 kD COMPARTMENT: cytoplasm cell nucleus MOTIF: Ser phosphorylation site {S43} alanine-rich region {206-221} MOTIF: alanine residue (SEVERAL) proline-rich region SITE: 243-246 MOTIF: proline residue (SEVERAL) Ser phosphorylation site {S324} lysine-rich region {355-358} MOTIF: lysine residue (SEVERAL) Zn finger PHD-type NAME: Zn finger PHD-type SITE: 379-426 EFFECTOR-BOUND: Zn+2 glutamate-rich region {434-446} MOTIF: glutamate residue (SEVERAL) Zn finger PHD-type NAME: Zn finger PHD-type SITE: 456-503 EFFECTOR-BOUND: Zn+2 chromodomain NAME: chromodomain SITE: 494-594 FOR-BINDING-OF: heterochromatin chromodomain NAME: chromodomain SITE: 631-673 FOR-BINDING-OF: heterochromatin lysine-rich region {697-703} MOTIF: lysine residue (SEVERAL) Ser phosphorylation site {S713} helicase NAME: helicase SITE: 748-932 MOTIF: ATP-binding site NAME: ATP-binding site SITE: 761-768 DEAD/H box NAME: DEAD/H box SITE: 883-886 Helicase C-terminal {1064-1229} Ser phosphorylation site {S1361} Ser phosphorylation site {S1364} Ser phosphorylation site {S1367} Ser phosphorylation site {S1549} PCNT interaction {1566-1966} MOTIF: Ser phosphorylation site {S1601} Ser phosphorylation site {S1605}

Database Correlations

OMIM 602120 UniProt Q12873 PFAM correlations Entrez Gene 1107 Kegg hsa:1107 ENZYME 3.6.4.12

References

UniProt :accession Q12873