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chromatin assembly factor 1 (CAF-1)
Function:
1) chromatin assembly in DNA replication & DNA repair
2) assembles histone octamers onto replicating DNA in vitro
3) 1st step of nucleosome assembly process, bringing newly synthesized histones H3 & H4 to replicating DNA
4) histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete histone octamer
5) heterochromatin maintenance in proliferating cells
- brings newly synthesized CBX proteins to heterochromatic DNA replication foci (putative)
Structure:
- contains RBBP4, CHAF1A, CHAF1B
- only minor amounts of RBBP4 are complexed with CHAF1A & CHAF1B in G1 phase
General
molecular complex
Properties
SUBUNITS: chromatin assembly factor 1 subunit A
COMPARTMENT: cell nucleus
MOTIF: binding site
SITE: 1-314
FOR-BINDING-OF: chromobox protein homolog 1
MOTIF: binding site
SITE: 1-49
FOR-BINDING-OF: proliferating cell nuclear antigen
Ser phosphorylation site {S65}
Ser phosphorylation site {S123}
Ser phosphorylation site {S138}
Ser phosphorylation site {S141}
Ser phosphorylation site {S143}
Ser phosphorylation site {S206}
Ser phosphorylation site {S224}
PxVxL {233-246}
lysine-rich region {323-453}
MOTIF: lysine residue (SEVERAL)
glutamate-rich region {602-608}
MOTIF: glutamate residue (SEVERAL)
homodimerization {642-678}
binding site
SITE: 60-956
FOR-BINDING-OF: chromatin assembly factor 1 subunit B
MOTIF: Thr phosphorylation site {T722}
Ser phosphorylation site {S772}
Ser phosphorylation site {S775}
Thr phosphorylation site {T865}
Ser phosphorylation site {S873}
glutamate-rich region {905-911}
MOTIF: glutamate residue (SEVERAL)
Ser phosphorylation site {S951}
chromatin assembly factor 1 subunit B
COMPARTMENT: cytoplasm
cell nucleus
MOTIF: WD repeat {11-54}
WD repeat {64-103}
WD repeat {127-166}
WD repeat {169-208}
WD repeat {228-279}
WD repeat {299-340}
WD repeat {344-385}
Ser phosphorylation site {S409}
Thr phosphorylation site {T419}
Ser phosphorylation site {S429}
Thr phosphorylation site {T433}
Ser phosphorylation site {S458}
Thr phosphorylation site {T495}
Thr phosphorylation site {T509}
Thr phosphorylation site {T521}
Thr phosphorylation site {T531}
Ser phosphorylation site {S538}
histone-binding protein RBBP4
COMPARTMENT: cell nucleus
MOTIF: acetylation site
SITE: N-TERMINUS
EFFECTOR-BOUND: acetyl
WD repeat {122-155}
WD repeat {175-206}
WD repeat {225-256}
WD repeat {271-302}
WD repeat {315-346}
WD repeat {372-403}
References
UniProt :accession Q13111, Q13112
Components
chromatin assembly factor 1 subunit A; CAF-1 subunit A; chromatin assembly factor I p150 subunit; CAF-I 150 kDa subunit; CAF-Ip150 (CHAF1A, CAF, CAF1P150)
chromatin assembly factor 1 subunit B; CAF-1 subunit B; chromatin assembly factor I p60 subunit; CAF-I 60 kD subunit; CAF-Ip60; M-phase phosphoprotein 7 (CHAF1B, CAF1A, CAF1P60, MPHOSPH7, MPP7)
histone-binding protein RBBP4; retinoblastoma-binding protein 4; RBBP-4; retinoblastoma-binding protein p48; chromatin assembly factor 1 subunit C; CAF-1 subunit C; chromatin assembly factor I p48 subunit; CAF-I 48 kD subunit; CAF-I p48; nucleosome-remodeling factor subunit RBAP48 (RBBP4, RbAp48)