charged multivesicular body protein 4A; chromatin-modifying protein 4a; CHMP4a; SNF7 homolog associated with Alix-2; SNF7-1; hSnf-1; vacuolar protein sorting-associated protein 32-1; Vps32-1; hVps32-1 (CHMP4A, C14orf123, SHAX2, CDA04, HSPC134)

Function: - component of ESCRT3 in the multivesicular body pathway - within ESCRT3, serves as an acceptor for ESCRT1 complex - interacts with CHMP2A, CHMP4B, CHMP4C, CHMP6 within ESCRT3 - interacts with ESCRT1 component PDCD6IP - self-associates - overexpression leads to the assembly of filaments that curve & associate to create circular rings that can promote outward budding - interacts with VPS4A Structure: - the acidic C-terminus & the basic N-termminus are thought to render the protein in a closed, soluble & inactive conformation through an autoinhibitory intramolecular interaction - the open & active conformation, which enables membrane binding & oligomerization, is facilitated by interaction with other ESCRT components (putative) - belongs to the SNF7 family Compartment: - cytoplasmic, membrane-associated - localizes to large vesicle-like structures - localizes to the midbody of dividing cells - localized in two distinct rings on either side of the midbody Expression: - widely expressed - expressed in heart, kidney, liver, skeletal muscle > brain, placenta, lung, pancreas Pathology: - role in HIV-1 virus release via its interaction with PDCD6IP

Properties

SIZE: entity length = 222 aa MW = 25 kD COMPARTMENT: cytoplasm MOTIF: Intramolecular interaction with C- terminus {1-150} MOTIF: phosphoinosides interaction {1-116} coiled coil {20-105} Intramolecular interaction with N- terminus {151-222} MOTIF: coiled coil {155-180}

Database Correlations

OMIM 610051 UniProt Q9BY43 Pfam PF03357 Entrez Gene 29082 Kegg hsa:29082

References

UniProt :accession Q9BY43