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chloride (Cl-) in body fluid

Reference values: - see chloride in serum Clinical significance: - see chloride in serum Principle: The Kodak Ektachem Clinical Chemistry Slide (CL-) is a dry, multilayered, analytical element that uses direct potentiometry for measurement of chloride ions. The slide consists of two ion-selective electrodes, each containing a protective layer, a silver & silver chloride layer coated on a plastic support. The protective layer inhibits interference from normal levels of bromide & uric acid. Application of 10 uL of sample & 10 uL of the Kodak Ektachem Electrolyte Reference Fluid to separate halves of the slide results in migration of both fluids towards the center of the paper bridge. A stable liquid junction is formed connecting the reference electrodes to the sample indicator electrode. Each electrode produces an electrical potential in response to the activity of chloride ions applied to it. The potential difference poised between the two electrodes is proportional to the chloride concentration in the sample. Specimen: No special patient preparation is necessary. Sample Preparation: Collect specimen by standard venipuncture technique. Do not draw specimen from an arm receiving intravenous transfusion. Heparin may be used as an anticoagulant for plasma specimens. Potassium oxalate/sodium fluoride should not be used as an anticoagulant. Remove serum or plasma promptly from the clot or cells. Handle specimens in stoppered containers to avoid contamination & evaporation. Refrigerate specimens at 2-8 degrees C if analysis is not performed within four hours. Freeze specimens at -18 degrees C if analysis is delayed beyond 48 hours. Minimum sample size 0.5 milliliter with an optimum size of 1.0 milliliter or larger. Interferences: 1) Preliminary data indicated that hemolysis & icterus do not interfere with this method. 2) Lipemic specimens show approximately the same positive bias versus coulometry as reported by Steffers & Frier (1976), due to ion-selective electrodes responding to the chloride concentration in serum water.

Related

Cl- (chloride) hyperchloremia hypochloremia

Specific

chloride (Cl-) in saliva chloride (Cl-) in serum/plasma chloride (Cl-) in sweat chloride (Cl-) in urine chloride in amniotic fluid chloride in CSF chloride in dialysis fluid chloride in gastric fluid chloride in milk chloride in pleural fluid chloride in serum/plasma/blood chloride in stool chloride in vitreous fluid

General

chloride (Cl-) measurement

References

  1. Kodak Ektachem 700 Analyzer Operator's Manual, Kodak Clinical Products, Rochester, New York.
  2. Kodak Ektachem Slide Package Inserts, Kodak Clinical Products Rochester, New York.
  3. Kodak Ektachem Training Manual, Kodak Clinical Products, Rochester, New York.
  4. Clinical Diagnosis & Management by Laboratory Methods, 19th edition, J.B. Henry (ed), W.B. Saunders Co., Philadelphia, PA. 1996, pg 10.
  5. Chloride, Body Fluid Laboratory Test Directory ARUP: 20163