centromere protein-E (CENP-E, CENPE)

Function: - minus-end directed microtubule motor - probable kinetochore motor - accumulates just before mitosis at the G2 phase of the cell cycle - probably important for chromosome movement &/or spindle elongation - interacts with CENP-F, BUBR1 kinase & PRC1 Structure: - belongs to the kinesin-like protein family - contains 1 kinesin-motor domain Compartment: - kinetochore, spindle, centromere - associates with kinetochores during congression - relocates to the spindle midzone at anaphase - quantitatively discarded at the end of cell division Alternative splicing: named isoforms=3

General

adenosine triphosphatase (ATPase) guanosine triphosphatase (GTPase) prenyl protein

Properties

SIZE: entity length = 2701 aa MW = 316 kD COMPARTMENT: cellular membrane MOTIF: kinesin motor domain SITE: 1-335 MOTIF: ATP-binding site NAME: ATP-binding site SITE: 86-93 binding site FOR-BINDING-OF: microtubule coiled coil {336-2143} MOTIF: Thr phosphorylation site {T422} Ser phosphorylation site {S454} Thr phosphorylation site {T544} Ser phosphorylation site {S611} Ser phosphorylation site {S1211} Thr phosphorylation site {T1268} coiled coil {2322-2490} Globular {2510-2701} MOTIF: Ser phosphorylation site {S2639} Ser phosphorylation site {S2651} Ser phosphorylation site {S2654} CaaX motif SITE: C-TERMINUS QUALITIES: AAX-TRUNCATED CARBOXYMETHYLATED EFFECTOR-BOUND: farnesyl BOUND-VIA: cysteine residue

Database Correlations

OMIM 117143 UniProt Q02224 Pfam PF00225 Kegg hsa:1062

References

UniProt :accession Q02224