cyclin-dependent kinase-7 (CDK7) or p40M015

Function: - CDK7 is the mammalian homolog to the Xenopus protein kinase MO15 - CDK7 is 1-2 subunits of CDK-activating kinase (CAK) along with cyclin-H - CAK is involved in controlling multiple cell cycle transitions phosphorylating many CDK/cyclin complexes - activation of CAK occurs through phosphorylation of Ser-170 & Thr-176 (presumably on CDK7) by another serine/threonine kinase - CAK is a component of TFIIH & in this form phosphorylates the C-terminal domain of the largest subunit of RNA polymerase 2 - activates the cyclin-associated kinases CDC2/CDK1, CDK2, CDK4 & CDK6 by Thr phosphorylation - involved in cell cycle control & in RNA transcription by RNA polymerase 2 - expression & activity are constant throughout the cell cycle - associates primarily with cyclin H & MAT1 to form the CAK complex - interacts with PUF60 - inactivated by phosphorylation - phosphorylation of Ser-164 during mitosis inactivates the enzyme - phosphorylation of Thr-170 is required for activity Structure: - belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily - contains 1 protein kinase domain Compartment: nucleus Expression: ubiquitous

Related

CDK7/cyclin H complex, CDK-activating kinase (CAK) or p34cdc2 activating kinase cyclin H (MO15-associated protein, CCNH)

Properties

SIZE: entity length = 346 aa MW = 39 kD COMPARTMENT: cell nucleus STATE: active state MOTIF: kinase domain SITE: 12-295 MOTIF: ATP-binding site NAME: ATP-binding site SITE: 18-26 ATP-binding site NAME: ATP-binding site SITE: 41-41 aspartate residue {D137} Ser phosphorylation site {S164} Thr phosphorylation site {T170} MISC-INFO: RECOGNITION-MOTIF :SEQUENCE RVYT*HEV :REF " - Kemp BE, Pearson RB. Protein kinase recognition sequence motifs. Trends Biochem Sci. 1990 Sep;15(9):342-6. Review. PMID: 2238044"

Database Correlations

OMIM 601955 UniProt P50613 Pfam PF00069 Entrez Gene 1022 Kegg hsa:1022 ENZYME correlations