Search
cyclin-dependent kinase-1 (CDK1), cell division control protein-2 (cdc2), p34 protein kinase or p34cdc2
Founding member of the cyclin-dependent kinase family.
Function:
- CDK1 is a component of MPF (Maturation Promoting Factor) & of CTD kinase
- role in the control of the eukaryotic cell cycle
- required in higher cells for entry into S-phase & mitosis
- p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase 2
- forms a stable but non-covalent complex with a regulatory subunit & with a cyclin
- interacts with DLG7
- isoform 2 is unable to complex with cyclin B1 & also fails to bind to the CDK inhibitor p21
- interacts with catalytically active CCNB1 & RALBP1 during mitosis to form an endocytotic complex during interphase
- phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme
- phosphorylation at Thr-161 activates it
- component of MPF (Maturation Promoting Factor)
- component of CTD kinase.
Structure:
- belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily
- contains 1 protein kinase domain
Compartment: nucleus (putative)
Alternative splicing: named isoforms=2
Pathology: found in breast cancer tissues
Comparative biology:
- Yeast homologs are cdc2 (S. cerevisiae), cdc28 (S. pombe), cdc2-kinase
- Thr-14 phosphorylation site is not present in yeast homologs
General
cyclin-dependent kinase (CDK)
Properties
SIZE: MW = 34 kD
STATE: Inactive
MOTIF: kinase domain
SITE: 4-287
MOTIF: ATP-binding site
NAME: ATP-binding site
SITE: 10-18
Thr phosphorylation site {T14}
Tyr phosphorylation site {Y15}
Tyr phosphorylation site {Y19}
ATP-binding site
NAME: ATP-binding site
SITE: 33-33
aspartate residue {D128}
Thr phosphorylation site {T161}
Database Correlations
OMIM 116940
UniProt P06493
Pfam PF00069
Entrez Gene 983
Kegg hsa:983
ENZYME correlations
References
- Gould KL, Moreno S, Tonks NK, Nurse P.
Complementation of the mitotic activator, p80cdc25, by a human
protein-tyrosine phosphatase.
Science. 1990 Dec 14;250(4987):1573-6.
PMID: 1703321
- De Bondt HL, Rosenblatt J, Jancarik J, Jones HD, Morgan DO,
Kim SH.
Crystal structure of cyclin-dependent kinase 2.
Nature. 1993 Jun 17;363(6430):595-602.
PMID: 8510751
{CDK2 crystal}
- Morgan DO.
Principles of CDK regulation.
Nature. 1995 Mar 9;374(6518):131-4. Review.
PMID: 7877684
Component-of
CDK1/cyclin B complex or maturation promoting factor (MPF)