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BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex (KLHL20)
Function:
- one of multiple E3 ubiquitin-protein ligase complexes formed of CUL3, RBX1 & variable BTB domain-containing proteins acting as both, adapter to cullin & substrate recognition
- the BCR complex may be active as a heterodimeric complex, in which NEDD8, covalently attached to one CUL3 molecule, binds to the C-terminus of a second CUL3 molecule
- mediates ubiquitination of
a) DAPK1, leading to its degradation by the proteasome, thus acting as a negative regulator of apoptosis
b) RHOA, leading to its degradation by the proteasome, thus regulating the actin cytoskeleton & cell migration
Structure:
- subunits: CUL3, KLHL20, RBX1, KCTD13, KLHL42
Notes:
- BCR complexes include:
a) BCR(SPOP) containing SPOP
b) BCR(KLHL9-KLHL13) complex with BTB domain proteins KLHL9 & KLHL13
c) the BCR(KLHL41) complex with BTB domain protein KLHL41
d) the BCR(KLHL42) complex with BTB domain protein KLHL42
e) BCR(KBTBD10) complex containing KBTBD10
f) BCR(ENC1) complex containing ENC1
General
molecular complex
E3 ubiquitin ligase; ubiquitin-ligating enzyme E3; N end-recognizing protein
Properties
MOTIF: active site
SUBUNITS: Cullin-3
COMPARTMENT: cell nucleus
golgi
MOTIF: Tyr phosphorylation site {Y58}
Ser phosphorylation site {S450}
Ser phosphorylation site {S737}
RING-box protein 1
COMPARTMENT: cytoplasm
cell nucleus
MOTIF: acetylation site
SITE: N-TERMINUS
EFFECTOR-BOUND: acetyl
Ser phosphorylation site {S11}
Zn+2-binding site
SITE: 42-42
Zn+2-binding site
SITE: 45-45
RING-finger {53-98}
MOTIF: Zn+2-binding site
SITE: 53-53
Zn+2-binding site
SITE: 56-56
Zn+2-binding site
SITE: 68-68
Zn+2-binding site
SITE: 75-75
Zn+2-binding site
SITE: 77-77
Zn+2-binding site
SITE: 80-80
Zn+2-binding site
SITE: 82-82
Zn+2-binding site
SITE: 83-83
Zn+2-binding site
SITE: 94-94
Zn+2-binding site
SITE: 97-97
EFFECTOR-BOUND: Zn+2
FOR-BINDING-OF: DNA motif
kelch-like protein 20
COMPARTMENT: cytoplasm
cell nucleus
MOTIF: BTB domain {68-135}
MOTIF: Zinc finger
NAME: Zinc finger
EFFECTOR-BOUND: Zn+2
BACK {170-272}
kelch repeat {319-365}
kelch repeat {367-413}
kelch repeat {414-460}
kelch repeat {462-507}
kelch repeat {509-554}
kelch repeat {556-601}
K+ channel tetramerization domain containing protein 13
COMPARTMENT: cell nucleus
MOTIF: BTB domain {41-109}
MOTIF: Zinc finger
NAME: Zinc finger
EFFECTOR-BOUND: Zn+2
References
- UniProt :accession Q13618
- UniProt :accession Q9Y2M5
- UniProt :accession Q8WZ19
Components
Cullin-3; CUL-3 (CUL3 KIAA0617)
K+ channel tetramerization domain containing protein 13; BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 1; hBACURD1; BTB/POZ domain-containing protein KCTD13; polymerase delta-interacting protein 1; TNFAIP1-like protein (KCTD13, BACURD1, PDIP1, POLDIP1, FKSG86, PP6832)
kelch-like protein 20; kelch-like ECT2-interacting protein; kelch-like protein X (KLHL20, KLEIP)
RING-box protein 1; Rbx1; regulator of cullins 1; RING finger protein 75; protein ZYP (RBX1 RNF75 ROC1)