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BRCA1-associated genome surveillance complex (BASC)
Function:
- role as a sensor of abnormal DNA structures &/or as a regulator of post-replication repair processes (putative)
Structure:
- large molecular complex which includes tumor suppressors & DNA repair proteins
- subunits: BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 & the RAD50-MRE11-NBS1 complex
General
molecular complex
Properties
SUBUNITS: BRCA1 protein
COMPARTMENT: cell nucleus
MOTIF: Zn finger C4-type
SITE: 24-64
EFFECTOR-BOUND: Zn+2
phosphorylation site
BRCA1 C-terminal (BRCT) motif
DNA mismatch repair protein Msh2
MOTIF: EXO1 interaction {601-671}
ATP-binding site
NAME: ATP-binding site
SITE: 669-676
Ser phosphorylation site {S860}
DNA mismatch repair protein Msh6
COMPARTMENT: cell nucleus
MOTIF: Ser phosphorylation site {S14}
alanine-rich region {34-37}
MOTIF: alanine residue (SEVERAL)
Ser phosphorylation site {S41}
Ser phosphorylation site {S43}
Ser phosphorylation site {S79}
Ser phosphorylation site {S91}
PWWP domain {92-154}
MOTIF: Ser phosphorylation site {S137}
Thr phosphorylation site {T139}
Ser phosphorylation site {S200}
glutamate-rich region {201-209}
MOTIF: glutamate residue (SEVERAL)
Ser phosphorylation site {S219}
Ser phosphorylation site {S227}
Ser phosphorylation site {S252}
Ser phosphorylation site {S254}
Ser phosphorylation site {S256}
Ser phosphorylation site {S261}
Ser phosphorylation site {S309}
Ser phosphorylation site {S348}
Ser phosphorylation site {S830}
Thr phosphorylation site {T924}
glutamate-rich region {1118-1123}
MOTIF: glutamate residue (SEVERAL)
ATP-binding site
NAME: ATP-binding site
SITE: 1134-1141
DNA mismatch repair protein Mlh1
MOTIF: EXO1 interaction {410-650}
MOTIF: Ser phosphorylation site {S477}
ATM protein
MOTIF: Thr phosphorylation site {T72}
Ser phosphorylation site {S85}
Thr phosphorylation site {T86}
Ser phosphorylation site {S367}
Thr phosphorylation site {T373}
interaction with ABL1 {1373-1382}
FAT domain
NAME: FAT domain
SITE: 1960-2566
MOTIF: Tyr phosphorylation site
binding site
FOR-BINDING-OF: Paxillin
Ser phosphorylation site {S1981}
Thr phosphorylation site {T1985}
PI3K/PI4K {2712-2962}
FATC {3024-3056}
STATE: active state
BLM protein
MOTIF: Ser phosphorylation site {S48}
Ser phosphorylation site {S53}
Thr phosphorylation site {T57}
Thr phosphorylation site {T114}
serine-rich region {310-316}
MOTIF: serine residue (SEVERAL)
Ser phosphorylation site {S358}
Ser phosphorylation site {S419}
Ser phosphorylation site {S422}
Ser phosphorylation site {S480}
Ser phosphorylation site {S499}
Thr phosphorylation site {T508}
helicase
NAME: helicase
SITE: 676-851
MOTIF: ATP-binding site
NAME: ATP-binding site
SITE: 689-696
DEAD/H box
NAME: DEAD/H box
SITE: 795-798
Helicase C-terminal {877-1024}
HRDC {1212-1292}
Ser phosphorylation site {S1295}
Ser phosphorylation site {S1296}
Ser phosphorylation site {S1303}
Ser phosphorylation site {S1310}
nuclear translocation signal {1334-1349}
nibrin
MOTIF: forkhead-associated (FHA) domain {24-83}
BRCA1 C-terminal (BRCT) motif
SITE: 105-181
Ser phosphorylation site {S278}
Thr phosphorylation site {T337}
Ser phosphorylation site {S341}
Ser phosphorylation site {S343}
Ser phosphorylation site {S397}
Ser phosphorylation site {S432}
glutamine-rich region {448-451}
MOTIF: glutamine residue (SEVERAL)
nuclear translocation signal {461-467}
Thr phosphorylation site {T602}
Ser phosphorylation site {S615}
EEXXXDDL {736-743}
double-strand break repair protein MRE11A
COMPARTMENT: cell nucleus
MOTIF: Thr phosphorylation site {T74}
histidine residue {H129}
Ser phosphorylation site {S649}
Ser phosphorylation site {S678}
Ser phosphorylation site {S688}
Ser phosphorylation site {S689}
DNA repair protein RAD50
COMPARTMENT: cell nucleus
MOTIF: ATP-binding site
NAME: ATP-binding site
SITE: 36-43
coiled coil {228-359}
MOTIF: Ser phosphorylation site {S237}
coiled coil {401-598}
Zinc-hook {635-734}
MOTIF: coiled coil {635-673}
Ser phosphorylation site {S635}
Ser phosphorylation site {S640}
Zn+2-binding site
SITE: 681-681
Zn+2-binding site
SITE: 684-684
Thr phosphorylation site {T690}
coiled coil {706-734}
coiled coil {789-1079}
replication factor-C
References
- Wang Y et al.
BASC, a super complex of BRCA1-associated proteins involved
in the recognition and repair of aberrant DNA structures.
Genes & Development 14:927-39, 2000
PMID: 10783165
Components
ATM protein (Ataxia Telangiectasia mutated)
Bloom syndrome protein; recQ protein-like 3; DNA helicase, recQ-like type 2 (BLM, RECQ2, RECQL3)
breast cancer type 1 susceptibility protein (BRCA1)
DNA mismatch repair protein Mlh1; mutL protein homolog 1 (MLH1, COCA2)
DNA mismatch repair protein Msh2; hMSH2; mutS protein homolog 2 (MSH2)
DNA mismatch repair protein Msh6; hMSH6; mutS-alpha 160 kD subunit; p160; G/T mismatch-binding protein; GTMBP; GTBP (MSH6 GTBP)
DNA repair protein RAD50; hRAD50 (RAD50)
double-strand break repair protein MRE11A; meiotic recombination 11 homolog A; MRE11 homolog A; meiotic recombination 11 homolog 1; MRE11 homolog 1 (MRE11A, HNGS1, MRE11)
nibrin; Nijmegen breakage syndrome protein 1; cell cycle regulatory protein p95 (NBN, NBS, NBS1, P95)
replication factor-C