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BRCA1-A complex

Function: - specifically recognizes Lys-63-linked ubiquitinated histones H2A & H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs) - BRCC3 specifically removes Lys-63-linked ubiquitin on histones H2A & H2AX within the complex, antagonizing RNF8-dependent ubiquitination at double-strand breaks Structure: - subunits: - BRCA1, BARD1, UIMC1/RAP80, FAM175A/Abraxas, BRCC3/BRCC36, BRE/BRCC45 & MERIT40/NBA1

Related

breast cancer type 1 susceptibility protein (BRCA1)

General

molecular complex

Properties

SUBUNITS: BRCA1 protein COMPARTMENT: cell nucleus MOTIF: Zn finger C4-type SITE: 24-64 EFFECTOR-BOUND: Zn+2 phosphorylation site BRCA1 C-terminal (BRCT) motif BARD1 protein COMPARTMENT: cytoplasm cell nucleus MOTIF: BRCA1 interaction {26-119} MOTIF: RING-finger {50-87} EFFECTOR-BOUND: Zn+2 FOR-BINDING-OF: DNA motif serine-rich region {400-403} MOTIF: serine residue (SEVERAL) ankyrin repeat NAME: ankyrin repeat SITE: 427-459 ankyrin repeat NAME: ankyrin repeat SITE: 460-492 ankyrin repeat NAME: ankyrin repeat SITE: 493-525 BRCA1 C-terminal (BRCT) motif SITE: 560-653 BRCA1 C-terminal (BRCT) motif SITE: 667-777 retinoid X receptor-interacting protein 110 COMPARTMENT: cell nucleus MOTIF: silencer element SITE: 1-101 MOTIF: Ser phosphorylation site {S44} Ser phosphorylation site {S46} LR {60-78} UIM {79-96} glutamate-rich region {81-108} MOTIF: glutamate residue (SEVERAL) UIM-linker {97-103} NR6A1 interaction {100-200} MOTIF: Ser phosphorylation site {S101} UIM {104-124} Ser phosphorylation site {S140} Ser phosphorylation site {S205} AIR {270-400} NR6A1 interaction {400-500} MOTIF: Ser phosphorylation site {S402} Ser phosphorylation site {S415} Ser phosphorylation site {S419} Zinc-finger-like region {505-582} Ser phosphorylation site {S597} Ser phosphorylation site {S627} Ser phosphorylation site {S653} Ser phosphorylation site {S677} Lys-63-specific deubiquitinase BRCC36 COMPARTMENT: cell nucleus MOTIF: acetylation site SITE: N-TERMINUS EFFECTOR-BOUND: acetyl peptide motif {122-135} MOTIF: Zn+2-binding site SITE: 122-122 Zn+2-binding site SITE: 124-124 Zn+2-binding site SITE: 135-135 Coiled-coil domain-containing protein 98 MOTIF: coiled coil {206-260} Thr phosphorylation site {T365} BRCA1-A complex subunit BRE COMPARTMENT: cytoplasm cell nucleus MOTIF: acetylation site SITE: N-TERMINUS EFFECTOR-BOUND: acetyl Ser phosphorylation site {S2} UEV-like 1 {30-147} UEV-like 2 {275-364}

References

UniProt :accession P46736

Components

BRCA1-A complex subunit BRE; BRCA1/BRCA2-containing complex subunit 45; brain & reproductive organ-expressed protein (BRE, BRCC45) BRCA1-associated RING domain protein 1 (BARD1) breast cancer type 1 susceptibility protein (BRCA1) Coiled-coil domain-containing protein 98; BRCA1-A complex subunit Abraxas (CCDC98, FAM175A, ABRA1, UNQ496/PRO1013) Lys-63-specific deubiquitinase BRCC36; BRCA1-A complex subunit BRCC36; BRISC complex subunit BRCC36; BRCA1/BRCA2-containing complex subunit 3; BRCA1/BRCA2-containing complex subunit 36 (BRCC3, BRCC36, C61A, CXorf53) retinoid X receptor-interacting protein 110; BRCA1-A complex subunit RAP80; receptor-associated protein 80; ubiquitin interaction motif-containing protein 1 (UIMC1, RAP80, RXRIP110)