Rho guanine nucleotide exchange factor 7; beta-Pix; COOL-1; PAK-interacting exchange factor beta; p85 (ARHGEF7, COOL1, KIAA0142, P85SPR, PAK3BP, PIXB, Nbla10314)

Function: - acts as a RAC1 guanine nucleotide exchange factor (GEF) - can induce membrane ruffling - may function as a positive regulator of apoptosis - may function in cell migration - downstream of NMDA receptors & CaMKK-CaMK1 signaling, promotes formation of spines & synapses in hippocampal neurons - phosphorylated on Ser-694 by CaMK1 - enhancement of GEF activity & downstream activation of RAC1 - interacts with PAK kinases through the SH3 domain - interacts with GIT1 & TGFB1I1 - interacts with ITCH - interacts with unphosphorylated PAK1 - interaction with SCRIB may play a role in regulation of apoptosis - interacts with FRMPD4 (via N-terminus) - interacts with CaMK1 Structure: - contains 1 CH (calponin-homology) domain - contains 1 DH (DBL-homology) domain - contains 1 PH domain - contains 1 SH3 domain Alternative splicing: named isoforms=6

General

phosphoprotein rho guanine nucleotide exchange factor

Properties

SIZE: entity length = 803 aa MW = 90 kD COMPARTMENT: cell nucleus MOTIF: calponin homology domain NAME: calponin homology domain SITE: 1-132 Ser phosphorylation site {S153} Ser phosphorylation site {S176} src homology 3 [SH3] domain SITE: 184-243 Ser phosphorylation site {S249} Dbl homology domain {271-451} PH domain {473-578} MOTIF: Ser phosphorylation site {S518} Ser phosphorylation site {S694}

Database Correlations

OMIM 605477 UniProt Q14155 PFAM correlations Entrez Gene 8874 Kegg hsa:8874

References

UniProt :accession Q14155