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bcl-xL

Function: - inhibits apoptosis through a bax-independent mechanism - associates with caspase 9 & APAF-1, inhibiting maturation of caspase 9 mediated by APAF-1 [4] - deamidation of bcl-xL with increased intracellular pH resulting from phosphorylation of NHE1 after DNA damage abrogates antiapoptic activity of bcl-xL [5] - overexpression leads to increased numbers of mature thymocytes Pathology: - BCR-ABL fusion gene may inhibit Bcl-xL deamidation in patients with CML [5] - JAK2 mutation may inhibit Bcl-xL deamidation in patients with polycythemia vera &/or myelofibrosis [5] Pharmacology: - UBX1325 binds to Bcl-xL & selectively induces apoptosis in senescent cells [6] - a single dose of UBX1325 improved visula acuity in patients with diabetic macular edema or neovascular age-related macular degeneration [6]

Interactions

molecular events

General

anti apoptotic protein Bcl-2-like protein 1; Bcl2-L-1; apoptosis regulator Bcl-X (BCL2L1, BCL2L, BCLX)

Properties

SIZE: entity length = 233 aa COMPARTMENT: mitochondria cytoplasm nuclear membrane MOTIF: BH4 domain NAME: BH4 domain SITE: S4-W24 BH3 domain NAME: BH3 domain SITE: V86-R100 BH1 domain NAME: BH1 domain SITE: E129-G148 BH2 domain NAME: BH2 domain SITE: P180-Y195 transmembrane domain {F210-L226}

References

  1. Boise LH et al bcl-x, a bcl-2-related gene that functions as a dominant regulator of apoptotic cell death. Cell. 1993 Aug 27;74(4):597-608. PMID: 8358789
  2. Cheng EH, Levine B, Boise LH, Thompson CB, Hardwick JM. Bax-independent inhibition of apoptosis by Bcl-XL. Nature. 1996 Feb 8;379(6565):554-6. PMID: 8596636
  3. Yang XF, Weber GF, Cantor H. A novel Bcl-x isoform connected to the T cell receptor regulates apoptosis in T cells. Immunity. 1997 Nov;7(5):629-39. PMID: 9390687
  4. Hu Y et al Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation. Proc Natl Acad Sci U S A. 1998 Apr 14;95(8):4386-91. PMID: 9539746
  5. Zhao R et al. Inhibition of the Bcl-xL deamidation pathway in myeloproliferative disorders. N Engl J Med 2008 Dec 25; 359:2778. PMID: 19109573
  6. Bankhead C 'Senolytic' Therapy Shows Promise in Neovascular Eye Disease. Clearing out dying cells, approach has implications far beyond ophthalmology. MedPage Today July 14, 2022 https://www.medpagetoday.com/meetingcoverage/asrs/99734

Component-of

molecular complex