sperm-associated antigen 5 (astrin, mitotic spindle-associated protein p126, MAP126, Deepest, SPAG5)

Function: - necessary for normal spindle formation during mitosis - homodimer, with a globular head domain & a long stalk - homo-oligomer; the globular head domains associate, resulting in aster-like structures - binds to microtubules in the mitotic spindle - phosphorylated upon DNA damage, probably by ATM or ATR Compartment: - cytoplasm, spindle, kinetochore - an a punctate pattern in interphase cells - during mitosis, detected at spindle poles during prophase, throughout the spindle in metaphase & anaphase, & at midzone microtubules in anaphase & telophase - detected on kinetochores of chromosomes that have congressed Expression: - highly expressed in testis - detected at lowlevels in placenta, liver, pancreas, thymus & colon

General

kinetochore protein phosphoprotein

Properties

SIZE: MW = 134 kD entity length = 1193 aa COMPARTMENT: cytoplasm MOTIF: Ser phosphorylation site {S12} Ser phosphorylation site {S14} Ser phosphorylation site {S62} Ser phosphorylation site {S66} Thr phosphorylation site {T111} Ser phosphorylation site {S135} Ser phosphorylation site {S249} Ser phosphorylation site {S334} Ser phosphorylation site {S341} Ser phosphorylation site {S353} Ser phosphorylation site {S362} Ser phosphorylation site {S364} Thr phosphorylation site {T377} Thr phosphorylation site {T389} Ser phosphorylation site {S401} coiled coil {545-608} glutamine-rich region {617-789} MOTIF: glutamine residue (SEVERAL) coiled coil {759-868} Thr phosphorylation site {T937} coiled coil {979-1174}

References

UniProt :accession Q96R06