Rho GTPase-activating protein 21 (Rho-type GTPase-activating protein 21, Rho GTPase-activating protein 10, ARHGAP21, ARHGAP10, KIAA1424)

Function: - functions as a GTPase-activating protein (GAP) for RHOA & CDC42 - downstream partner of ARF1 which may control Golgi structure & function - also required for CTNNA1 recruitment to adherens junctions - interacts with GTP-bound ARF1 & ARF6 - interacts with CTNNA1 - depletion of ARHGAP21 induces cell spreading & accumulation of F-actin stress fibers Structure: - contains 1 PDZ (DHR) domain - contains 1 PH domain - contains 1 Rho-GAP domain Compartment: - Golgi, peripheral membrane, cell junction cytoplasmic vesicle membrane - localization to Golgi is dependent on interaction with GTP-bound ARF1 Alternative splicing: named isoforms=3 Expression: - widely expressed - higher expression in brain, heart, skeletal muscle & placenta - up-regulated upon cell differentiation Pathology: - required for In1A-dependent entry of Listeria monocytogenes into cells

Properties

SIZE: entity length = 1957 aa MW = 217 kD MOTIF: Ser phosphorylation site {S35} PDZ domain NAME: PDZ domain SITE: 49-158 ARF1 & ARF6 interaction {929-1096} MOTIF: PH domain {930-1039} Ser phosphorylation site {S1089} Ser phosphorylation site {S1098} rho-GAP domain NAME: rho-GAP domain SITE: 1146-1338 MOTIF: Ser phosphorylation site {S1219} Ser phosphorylation site {S1220} Thr phosphorylation site {T1282} domain {C-TERMINAL} MOTIF: active site binding site FOR-BINDING-OF: rho subfamily protein HOMOLOGY: p160BCR beta-chimaerin n-chimaerin Rho-GTPase-activating protein 5 rho-GAP SH3 domain-binding protein 1 PI-3-kinase p85 drosophila rotund protein CTNNA1 interaction {1591-1860}

Database Correlations

UniProt Q5T5U3 PFAM correlations

References

UniProt :accession Q5T5U3