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ADP-ribosylation factor 1 (ARF1)
Function:
- GTP-binding protein
- role in protein trafficking among different compartments
- modulates vesicle budding & uncoating within the Golgi complex
- role in initial step of assembly & budding of COP-coated vesicles mediating intercisternal GOLGI transport
- deactivation induces redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking
- in its GTP-bound form, triggers association with coat proteins with the Golgi membrane
- hydrolysis of ARF1-bound GTP, mediated by ARFGAPs, is required for dissociation of coat proteins from Golgi membranes & vesicles
- interacts with PRKCABP, GGA1, ASAP2 & PIP5K1A
- ARFGAP1 hydrolyzes ARF1-GTP & regulates its function
- binds ASAP2
- interacts with HERC1 & ARHGAP21
Structure: belongs to the small GTPase superfamily, Arf family
Compartment: Golgi apparatus
Pathology:
- allosteric activator of the cholera toxin catalytic subunit
General
ADP-ribosylation factor [ARF] subfamily protein
Properties
SIZE: entity length = 181 aa
MW = 21 kD
COMPARTMENT: golgi
MOTIF: myristoylation site
SITE: G2
EFFECTOR-BOUND: myristate
GTP-binding site
SITE: 24-31
GTP-binding site
SITE: 126-129
Ser phosphorylation site {S147}
Database Correlations
OMIM 103180
UniProt P84077
Pfam PF00025
Entrez Gene 375
Kegg hsa:375
References
- UniProt :accession P84077
- Kahn RA, Der CJ, Bokoch GM.
The ras superfamily of GTP-binding proteins: guidelines on
nomenclature.
FASEB J. 1992 May;6(8):2512-3.
PMID: 1592203
- D'Souza-Schorey C, Li G, Colombo MI, Stahl PD.
A regulatory role for ARF6 in receptor-mediated endocytosis.
Science. 1995 Feb 24;267(5201):1175-8.
PMID: 7855600
- Entrez Gene :accession 375