apolipoprotein B100 (apoB100)

Function: - apo B100 is involved in lipid transport & clearance - ligand for the LDL receptor - apo B100 is the major inhibitor of membrane binding & pore insertion activity of perforin Structure: - recognition site for the LDL receptor is in the C-terminal portion of the protein Expression: - hepatic synthesis of apoB100 is about 10 mg/kg/day

Properties

SIZE: entity length = 4563 aa MW = 516 kD COMPARTMENT: plasma MOTIF: signal sequence {1-27} binding site SITE: 32-126 FOR-BINDING-OF: heparin MOTIF: N-glycosylation site {N34} cysteine residue {C39} MODIFICATION: cysteine residue {C88} Vitellogenin {46-672} MOTIF: cysteine residue {C78} MODIFICATION: cysteine residue {C97} cysteine residue {C88} MODIFICATION: cysteine residue {C39} cysteine residue {C97} MODIFICATION: cysteine residue {C78} N-glycosylation site {N185} cysteine residue {C186} MODIFICATION: cysteine residue {C212} cysteine residue {C212} MODIFICATION: cysteine residue {C186} binding site SITE: 232-306 FOR-BINDING-OF: heparin cysteine residue {C245} MODIFICATION: cysteine residue {C261} cysteine residue {C261} MODIFICATION: cysteine residue {C245} cysteine residue {C385} MODIFICATION: cysteine residue {C390} cysteine residue {C390} MODIFICATION: cysteine residue {C385} cysteine residue {C478} MODIFICATION: cysteine residue {C513} cysteine residue {C513} MODIFICATION: cysteine residue {C478} binding site SITE: 902-959 FOR-BINDING-OF: heparin MOTIF: Ser phosphorylation site {S928} cysteine residue {C966} MODIFICATION: cysteine residue {C976} cysteine residue {C976} MODIFICATION: cysteine residue {C966} N-glycosylation site {N983} cysteine residue {C1112} MODIFICATION: palmitate COMPARTMENT: membrane N-glycosylation site {N1368} N-glycosylation site {N1377} N-glycosylation site {N1523} binding site SITE: 2043-2178 FOR-BINDING-OF: heparin N-glycosylation site {N2239} binding site FOR-BINDING-OF: lipid N-glycosylation site {N2560} N-glycosylation site {N2779} N-glycosylation site {N2982} N-glycosylation site {N3101} binding site SITE: 3161-3236 FOR-BINDING-OF: heparin MOTIF: basic region {3174-3184} cysteine residue {C3194} MODIFICATION: cysteine residue {C3324} N-glycosylation site {N3224} cysteine residue {C3324} MODIFICATION: cysteine residue {C3194} N-glycosylation site {N3336} N-glycosylation site {N3358} LDL receptor binding {3373-3393} binding site SITE: 3383-3516 FOR-BINDING-OF: heparin MOTIF: basic region {3386-3394} N-glycosylation site {N3411} N-glycosylation site {N3465} N-glycosylation site {N3895} N-glycosylation site {N4237} N-glycosylation site {N4431} Ser phosphorylation site {S4507} MISC-INFO: 1/2life 2-5 DAYS