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anaphase promoting complex (APC, cyclosome)
Function:
- cell cycle-regulated E3 ubiquitin-protein ligase
- controls progression through mitosis & G1 phase of cell cycle
- promotes degradation of key mitotic regulatory proteins
- required for anaphase initiation & exit from mitosis.
- activated by cdc20 & HCDH1 which bind to & activate its
- cyclin ubiquitination activity
Structure:
- large 20S multiprotein complex
- composed of at least 11 subunits
General
molecular complex
Properties
SUBUNITS: anaphase promoting complex subunit 1
MOTIF: Ser phosphorylation site {S202}
Ser phosphorylation site {S286}
Thr phosphorylation site {T291}
Ser phosphorylation site {S355}
Ser phosphorylation site {S373}
Ser phosphorylation site {S377}
Thr phosphorylation site {T537}
Tyr phosphorylation site {Y571}
Ser phosphorylation site {S686}
Ser phosphorylation site {S688}
Ser phosphorylation site {S731}
proteasome/cyclosome repeat {1297-1325}
proteasome/cyclosome repeat {1366-1404}
proteasome/cyclosome repeat {1467-1501}
proteasome/cyclosome repeat {1520-1552}
anaphase promoting complex subunit 2
MOTIF: Ser phosphorylation site {S218}
Ser phosphorylation site {S314}
Ser phosphorylation site {S534}
Tyr phosphorylation site {Y810}
anaphase promoting complex subunit 4
MOTIF: Tyr phosphorylation site {Y469}
Ser phosphorylation site {S779}
anaphase promoting complex subunit 5
MOTIF: Ser phosphorylation site {S195}
Ser phosphorylation site {S217}
tetratricopeptide repeat {301-334}
tetratricopeptide repeat {522-555}
tetratricopeptide repeat {581-614}
tetratricopeptide repeat {678-711}
anaphase promoting complex subunit 6
MOTIF: Ser phosphorylation site {S112}
tetratricopeptide repeat {130-163}
tetratricopeptide repeat {299-333}
tetratricopeptide repeat {334-367}
tetratricopeptide repeat {368-401}
tetratricopeptide repeat {403-435}
tetratricopeptide repeat {445-478}
tetratricopeptide repeat {479-512}
MOTIF: Ser phosphorylation site {S490}
Ser phosphorylation site {S560}
Thr phosphorylation site {T581}
Ser phosphorylation site {S595}
Thr phosphorylation site {T599}
anaphase promoting complex subunit 7
MOTIF: tetratricopeptide repeat {43-76}
tetratricopeptide repeat {135-168}
tetratricopeptide repeat {237-270}
tetratricopeptide repeat {339-372}
tetratricopeptide repeat {407-441}
tetratricopeptide repeat {442-474}
tetratricopeptide repeat {475-508}
anaphase promoting complex subunit 8
MOTIF: tetratricopeptide repeat {77-110}
tetratricopeptide repeat {163-196}
tetratricopeptide repeat {222-255}
tetratricopeptide repeat {257-290}
MOTIF: Tyr phosphorylation site {Y267}
tetratricopeptide repeat {325-358}
tetratricopeptide repeat {360-392}
tetratricopeptide repeat {394-426}
tetratricopeptide repeat {427-460}
tetratricopeptide repeat {462-494}
Thr phosphorylation site {T556}
Thr phosphorylation site {T559}
Ser phosphorylation site {S572}
Ser phosphorylation site {S582}
Thr phosphorylation site {T590}
anaphase promoting complex subunit 10
MOTIF: DOC {1-185}
anaphase promoting complex subunit 11
MOTIF: Zn+2-binding site
SITE: 23-23
Zn+2-binding site
SITE: 26-26
RING-finger {34-77}
MOTIF: Zn+2-binding site
SITE: 34-34
Zn+2-binding site
SITE: 37-37
Zn+2-binding site
SITE: 44-44
Zn+2-binding site
SITE: 51-51
Zn+2-binding site
SITE: 53-53
Zn+2-binding site
SITE: 56-56
Zn+2-binding site
SITE: 58-58
Zn+2-binding site
SITE: 59-59
Zn+2-binding site
SITE: 73-73
Zn+2-binding site
SITE: 76-76
EFFECTOR-BOUND: Zn+2
FOR-BINDING-OF: DNA motif
Anaphase-promoting complex subunit 13
anaphase-promoting complex subunit CDC26
MOTIF: coiled coil {7-38}
Ser phosphorylation site {S78}
Ser phosphorylation site {S82}
Database Correlations
OMIM 603618
References
- Jallepalli & Lengauer.
Chromsosome segregation and cancer: cutting through the
mystery
Nature Reviews Cancer 1:109-117, 2001
PMID: 11905802
- OMIM :accession 603618
- UniProt :accession Q8NHZ8
Components
anaphase promoting complex subunit 1 (APC1, cyclosome subunit 1, protein Tsg24, testis-specific gene 24 protein, mitotic checkpoint regulator, ANAPC1, TSG24)
anaphase promoting complex subunit 10; cyclosome subunit 10 (APC10, ANAPC10)
anaphase promoting complex subunit 11; hepatocellular carcinoma associated ring finger protein; protein HSPC214 (ANAPC11)
anaphase promoting complex subunit 2 (APC2, ANAPC2, cyclosome subunit 2, KIAA1406)
anaphase promoting complex subunit 4 (APC4, ANAPC4, cyclosome subunit 4)
anaphase promoting complex subunit 5 (cyclosome subunit 5, ANAPC5, APC5)
anaphase promoting complex subunit 6 (cell division cycle protein 16 homolog, CDC16, CDC16Hs, APC6, cyclosome subunit 6)
anaphase promoting complex subunit 7 (APC7, ANAPC7, cyclosome subunit 7)
anaphase promoting complex subunit 8 (cell division cycle protein 23 homolog, CDC23, APC8, cyclosome subunit 8)
Anaphase-promoting complex subunit 13 (APC13) (Cylosome subunit 13) (ANAPC13)
anaphase-promoting complex subunit CDC26 (cell division cycle protein 26, CDC26)