amyloid

Pathology: - insoluble protein/peptide aggregates with a beta pleated sheet conformation - the beta-pleated sheet gives amyloid the characteristic insolubility & staining properties Microscopic appearance/staining: 1) hematoxylin & eosin: amorphous pink fibrillary aggregates 2) Congo red: apple green birefringence seen with polarizing filter, orange red color without polarizing filter 3) thioflavin T 4) crystal violet (type of methyl violet): metachromatic staining Laboratory: - amyloid in tissue by light microscopy - microscopic observation in tissue by congo red stain - beta-2-microglobulin amyloid Ag in tissue - Ig.kappa amyloid Ag in tissue - Ig.lambda amyloid Ag in tissue - amyloid P component Ag in tissue - amyloid A in tissue - amyloid A component Ag in tissue - amyloid precursor protein Ag in tissue - amyloid.prealbumin Ag in tissue

Specific

A4 amyloid peptide; beta-peptide ABri amyloid ADan amyloid AGel amyloid amylin; islet amyloid polypeptide; diabetes-associated peptide; DAP; insulinoma amyloid peptide (IAPP) cerebral amyloid gamma-trace Ig light chain amyloid non-A-beta component of AD amyloid serum amyloid A-2 protein (SAA2) serum amyloid A-4 protein; constitutively expressed serum amyloid A protein; C-SAA (SAA4 CSAA) serum amyloid A1 protein; SAA; contains: amyloid protein A; amyloid fibril protein AA (SAA1) serum amyloid P component; 9.5S alpha-1 glycoprotein (SAP)

References

Mayo Internal Medicine Board Review, 1998-99, Prakash UBS (ed) Lippincott-Raven, Philadelphia, 1998, pg 610