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amylase in body fluid

Clinical significance: - see amylase in serum, amylase in urine Principle: The kodak Ektachem Clinical Chemistry Slide (AMYL) is a dry multilayered, analytical element coated in a clear plastic support. The Kodak Ektachem Clinical Chemistry Slide (AMYL) quantitatively measures amylase activity in serum, plasma or urine. A 10 uL drop of patient sample is deposited on the slide. The porous spreading layer, which evenly distributes the sample, also contains the dyed starch substrate (Drimarene Red Z2B covalently linked to amylopectin) for the reaction. The amylase in the sample catalyzes the hydrolysis of this dyed starch into smaller dyed saccharides. These dyed saccharides diffuse into the underlying reagent layer that contains a cationic hydrophobic polymeric mordant, which binds the released anionic dye fragments and removes them from solution. At 2.3 minutes into the slide's total incubation period, the reflection density of the dye in the reagent layer is measured photometrically through the polyester slide support, using the white underside of the spreading layer as a diffuse reflector. Unreacted dye starch in the layer remains largely hidden from view. At the end of the incubation period, a second & final reflection density between the two readings is proportional to sample amylase activity. The following reaction sequences are involved: Amylase Dyed Starch -------------> Dyed Saccharides (high molecular weight) (low molecular weight) Specimen: No special patient preparation is necessary. See amylase in serum, amylase in urine Minimum sample size 0.5 milliliters with an optimum size of 1.0 milliliter or larger

Related

amylase hyperamylasemia

Specific

amylase in amniotic fluid amylase in CSF amylase in dialysis fluid amylase in duodenal fluid amylase in gastric fluid amylase in pericardial fluid amylase in peritoneal fluid amylase in pleural fluid amylase in saliva amylase in serum/plasma amylase in serum/plasma/blood amylase in synovial fluid amylase in urine amylase pancreatic in body fluid

General

general chemistry test

References

  1. Kodak Ektachem 700 Analyzer Operator's Manual, Kodak Clinical Products, Rochester, New York.
  2. Kodak Ektachem Slide Package Inserts, Kodak Clinical Products Rochester, New York.
  3. Kodak Ektachem Training Manual, Kodak Clinical Products, Rochester, New York.
  4. Clinical Diagnosis & Management by Laboratory Methods, 19th edition, J.B. Henry (ed), W.B. Saunders Co., Philadelphia, PA. 1996, pg 10.
  5. Amylase, Body Fluid Laboratory Test Directory ARUP: 20506