amylase-2A (pancreatic amylase-A, 1,4-alpha-D- glucan glucanohydrolase, AMY2A)

Function: - endohydrolysis of 1,4-alpha-D-glucosidic linkages in oligosaccharides & polysaccharides Cofactor: - binds 1 Ca⁺² per subunit - binds 1 chloride per subunit Structure: - monomer - belongs to the glycosyl hydrolase 13 family Compartment: secreted, extracellular space

General

amylase-2 or pancreatic amylase glycoprotein

Properties

SIZE: entity length = 511 aa MW = 58 kD COMPARTMENT: extracellular compartment MOTIF: signal sequence {1-15} cysteine residue {C43} MODIFICATION: cysteine residue {C101} cysteine residue {C85} MODIFICATION: cysteine residue {C130} cysteine residue {C101} MODIFICATION: cysteine residue {C43} Ca+2-binding site SITE: 115-115 cysteine residue {C130} MODIFICATION: cysteine residue {C85} cysteine residue {C156} MODIFICATION: cysteine residue {C175} Ca+2-binding site SITE: 173-173 cysteine residue {C175} MODIFICATION: cysteine residue {C156} Ca+2-binding site SITE: 182-182 binding site SITE: 210-210 FOR-BINDING-OF: Chloride aspartate residue {D212} Ca+2-binding site SITE: 216-216 glutamate residue {E248} binding site SITE: 313-313 FOR-BINDING-OF: Chloride aspartate residue {D315} binding site SITE: 352-352 FOR-BINDING-OF: Chloride cysteine residue {C393} MODIFICATION: cysteine residue {C399} cysteine residue {C399} MODIFICATION: cysteine residue {C393} cysteine residue {C465} MODIFICATION: cysteine residue {C477} N-glycosylation site {N476} cysteine residue {C477} MODIFICATION: cysteine residue {C465}

Database Correlations

OMIM 104650 UniProt P04746 PFAM correlations Entrez Gene 279 ENZYME 3.2.1.1