angiomotin (AMOT, KIAA1071)

Function: - role in maintenance of tight junctions via complex with ARHGAP17, which acts by regulating uptake of polarity proteins at tight junctions - appears to regulate endothelial cell migration & tube formation (blood vessel formation) - may also play a role in assembly of endothelial cell- cell junctions - phosphorylated upon DNA damage, probably by ATM or ATR - polyubiquitinated by NEDD4, NEDD4L & ITCH, leading to proteasomal degradation - component of a complex whose core is composed of ARHGAP17, AMOT, MPP5/PALS1, INADL/PATJ & PARD3/PAR3 - interacts with MAGI1 - isoform 1 interacts with angiostatin Structure: - the coiled coil domain interacts directly with the BAR domain of ARHGAP17 - the angiostatin binding domain (871-1005) allows binding to angiostatin - belongs to the angiomotin family Compartment: - cell junction, tight junction - localized on the cell surface - may act as a transmembrane protein Alternative splicing: named isoforms=2 Expression: - expressed in placenta, skeletal muscle, endothelial cells of capillaries & larger vessels of placenta Notes: 'Motus' means 'motility' in latin

General

membrane protein phosphoprotein

Properties

SIZE: entity length = 1084 aa MW = 118 kD COMPARTMENT: cellular membrane MOTIF: Ser phosphorylation site {S305} Ser phosphorylation site {S312} coiled coil {429-689} Ser phosphorylation site {S712} Ser phosphorylation site {S714} Thr phosphorylation site {T717} Tyr phosphorylation site {Y719} coiled coil {721-751} Thr phosphorylation site {T1061} PDZ recognition motif NAME: PDZ recognition motif SITE: 1081-1084 FOR-BINDING-VIA: PDZ domain

Database Correlations

OMIM 300410 UniProt Q4VCS5 Pfam PF12240 Entrez Gene 154796 Kegg hsa:154796

References

UniProt :accession Q4VCS5